สืบค้นงานวิจัย
Molecular identification and genetic variation of hematophagous flies, (Diptera: Muscidae: Stomoxyinae) in Thailand based on cox1 barcodes
Changbunjong T. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Molecular identification and genetic variation of hematophagous flies, (Diptera: Muscidae: Stomoxyinae) in Thailand based on cox1 barcodes
ผู้แต่ง / หัวหน้าโครงการ (EN): Changbunjong T.
บทคัดย่อ (EN): Stomoxyinae flies (Diptera: Muscidae) transmit many pathogens to animals and humans. However, morphological identification of Stomoxyinae flies is difficult, and there is risk of misidentification. Therefore, this study analyzed cytochrome oxidase 1 (cox1) barcodes to test their ability to discriminate between taxonomic groups. cox1 barcodes (657 bp) from 66 specimens were classified into 8 species. cox1 sequences have typical insect mitochondrial characteristics including a high AT content (mean = 69%), especially at the third codon position. cox1 barcodes were able to discriminate between species with high interspecific divergence, especially those that are morphologically difficult to distinguish, such as certain Stomoxys species (S. indicus, S. pullus, and S. uruma) and Haematobia sub-species (H. irritans irritans and H. irritans exigua). Phylogenetic analysis and the genealogical sorting index revealed that all Stomoxyinae species formed a monophyletic group and were clearly separated into distinct clusters based on species. Species delimitation by the Poisson tree processes model indicated previously unrecognized diversity within Stomoxys and Haematobosca. Thus, DNA barcoding was able to discriminate between morphologically uncertain or misidentified Stomoxyinae flies and confirm the correct species, which are important steps for elucidating diversity. © 2016 Korean Society of Applied Entomology, Taiwan Entomological Society and Malaysian Plant Protection Society.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-84992679759&doi=10.1016%2fj.aspen.2016.10.006&partnerID=40&md5=7768c98561973c034dc3ea8733a66411
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Stomoxyinae
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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Molecular identification and genetic variation of hematophagous flies, (Diptera: Muscidae: Stomoxyinae) in Thailand based on cox1 barcodes
Changbunjong T.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
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