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Separating of bovine seminal plasma proteins by using 2D-PAGE revealed the different profile of PDC-109
T. Thepparat - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Separating of bovine seminal plasma proteins by using 2D-PAGE revealed the different profile of PDC-109
ผู้แต่ง / หัวหน้าโครงการ (EN): T. Thepparat
บทคัดย่อ (EN): Several lines of evidence showed that some proteins in seminal plasma were correlated to bull fertility and can be used as the potential protein markers for identifying the fertile bulls. This preliminary study aimed to find out any different expression proteins in seminal plasma of the bulls by using proteomic approach. Seminal plasma proteinsfrom 4 Brahman bulls, 3-5 years of age, were separated using 2D-PAGE followed by staining with Coomassie blue. At least a triplicate gels was performed for each sample. Each gel was scanned with an ImageScanner System and analyzed for spots by ImageMasterTM 2D Platinum software. The different expression protein spot(s) was cut from the gel and identified by LC MS/MS. The results showed that at least 200 protein spots were detected in the seminal plasma of the Brahman bulls. Of all these spots, the relative protein content of2 spots at 16.5 kDa, pI 5.3 and 5.5 expressed differently among 4 bulls. Both spots were identified as PDC-109. It was concluded that by using 2D-PAGE, PDC-109 was a different expression protein in seminal plasma of the Brahman bulls. Further study of this protein in relation to bull fertility may lead to one promising marker for selecting the fertile bull.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): th
จำนวนหน้า: 5
เอกสารแนบ: https://ag2.kku.ac.th/kaj/PDF.cfm?filename=831.pdf&id=750&keeptrack=14
คำสำคัญ (EN): PDC-109
หากไม่พบเอกสารฉบับเต็ม (Full Text) โปรดติดต่อหน่วยงานเจ้าของข้อมูล

การอ้างอิง

T. Thepparat. "Separating of bovine seminal plasma proteins by using 2D-PAGE revealed the different profile of PDC-109". ไม่ระบุหน่วยงาน. 2555

T. Thepparat. "Separating of bovine seminal plasma proteins by using 2D-PAGE revealed the different profile of PDC-109". ไม่ระบุหน่วยงาน. 2555. Print.



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Separating of bovine seminal plasma proteins by using 2D-PAGE revealed the different profile of PDC-109
T. Thepparat
ไม่ระบุผู้เผยแพร่
2555
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