สืบค้นงานวิจัย
Immunochemical characterization of purified buffalo beta-lactoglobulin (Buffalo Bulletin (Thailand),35(2))
Siddhartha Narayan Joardar - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Immunochemical characterization of purified buffalo beta-lactoglobulin (Buffalo Bulletin (Thailand),35(2))
ผู้แต่ง / หัวหน้าโครงการ (EN): Siddhartha Narayan Joardar
หน่วยงานสังกัดผู้แต่ง (EN): Kolkata (India)
บทคัดย่อ (EN): Beta-lactoglobulin (β-lg) is a major whey protein found in cow milk and other ruminants, deer, bison, and buffalo. β-lg is considered as one of the main allergenic components in bovine milk and therefore the modification of β-lg is considered as a promising treatment for milk allergy. Thus the present study was conducted to assess the antigenicity of purified β-lg that can be exploited for detection of buffalo milk-protein intolerance. β-lg was purified from milk of Murrah buffalo of Haringhata Farm, West Bengal (India) by combination of gel filtration (using Sephacryl S-200) and anion-exchange chromatography (using DEAE-Sepharose). The molecular weight was derived as 18.05 kDa in 15 percent one-dimensional SDS-PAGE. Antigenicity (seroreactivity) of purified β-lg was evaluated by double immuno diffusion (DID), western blotting, dipstick ELISA and indirect ELISA using hyperimmune sera raised in rabbit against purified buffalo β-lg. In indirect ELISA, titer of rabbit serum against buffalo β-lg was observed to be in between 200-400. Therefore, the study revealed that purified buffalo β-lg is highly antigenic and a seroreactive protein and may be used for detection of buffalo milk proteinintolerance using rapid, user-friendly, cost effective sero-diagnostic technique/tool.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://kukr.lib.ku.ac.th/db/kukr/search_detail/result/344238
คำสำคัญ (EN): Dipstick ELISA
หากไม่พบเอกสารฉบับเต็ม (Full Text) โปรดติดต่อหน่วยงานเจ้าของข้อมูล

การอ้างอิง

Siddhartha Narayan Joardar. "Immunochemical characterization of purified buffalo beta-lactoglobulin (Buffalo Bulletin (Thailand),35(2))". ไม่ระบุหน่วยงาน. ม.ป.ป.

Siddhartha Narayan Joardar. "Immunochemical characterization of purified buffalo beta-lactoglobulin (Buffalo Bulletin (Thailand),35(2))". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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Immunochemical characterization of purified buffalo beta-lactoglobulin (Buffalo Bulletin (Thailand),35(2))
Siddhartha Narayan Joardar
ไม่ระบุผู้เผยแพร่
ไม่ระบุวันที่เผยแพร่
Hydroallantois in a buffalo with fetal anasarca (Buffalo Bulletin (Thailand),35(4)) Endoscopic diagnosis of megaesophagus in a buffalo (Buffalo Bulletin (Thailand),35(2)) Bilateral uncomplicated anopthalmia in a buffalo calf (Buffalo Bulletin (Thailand),35(4)) Production performance of Nagpuri buffalo under field condition (Buffalo Bulletin (Thailand),35(4)) Dicephalous dipus tetrabrachius calf in a Murrah buffalo (Buffalo Bulletin (Thailand),35(4)) Septicemia in a Murrah buffalo calf: A case report (Buffalo Bulletin (Thailand),35(4)) Quality characteristics of fermented buffalo beef sausage (Buffalo Bulletin (Thailand),35(4)) Ovarian teratoma in a water buffalo (Bubalus bubalis) (Buffalo Bulletin (Thailand),35(4)) Dystocia due to rare foetal monster in a buffalo (Buffalo Bulletin (Thailand),35(4)) Confirmation of rabies in buffalo from organized farm of north Gujarat (Buffalo Bulletin (Thailand),35(4))
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