สืบค้นงานวิจัย
Immunochemical characterization of purified buffalo beta-lactoglobulin (Buffalo Bulletin (Thailand),35(2))
Siddhartha Narayan Joardar - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Immunochemical characterization of purified buffalo beta-lactoglobulin (Buffalo Bulletin (Thailand),35(2))
ผู้แต่ง / หัวหน้าโครงการ (EN): Siddhartha Narayan Joardar
หน่วยงานสังกัดผู้แต่ง (EN): Kolkata (India)
บทคัดย่อ (EN): Beta-lactoglobulin (β-lg) is a major whey protein found in cow milk and other ruminants, deer, bison, and buffalo. β-lg is considered as one of the main allergenic components in bovine milk and therefore the modification of β-lg is considered as a promising treatment for milk allergy. Thus the present study was conducted to assess the antigenicity of purified β-lg that can be exploited for detection of buffalo milk-protein intolerance. β-lg was purified from milk of Murrah buffalo of Haringhata Farm, West Bengal (India) by combination of gel filtration (using Sephacryl S-200) and anion-exchange chromatography (using DEAE-Sepharose). The molecular weight was derived as 18.05 kDa in 15 percent one-dimensional SDS-PAGE. Antigenicity (seroreactivity) of purified β-lg was evaluated by double immuno diffusion (DID), western blotting, dipstick ELISA and indirect ELISA using hyperimmune sera raised in rabbit against purified buffalo β-lg. In indirect ELISA, titer of rabbit serum against buffalo β-lg was observed to be in between 200-400. Therefore, the study revealed that purified buffalo β-lg is highly antigenic and a seroreactive protein and may be used for detection of buffalo milk proteinintolerance using rapid, user-friendly, cost effective sero-diagnostic technique/tool.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://kukr.lib.ku.ac.th/db/kukr/search_detail/result/344238
คำสำคัญ (EN): Dipstick ELISA
หากไม่พบเอกสารฉบับเต็ม (Full Text) โปรดติดต่อหน่วยงานเจ้าของข้อมูล

การอ้างอิง

Siddhartha Narayan Joardar. "Immunochemical characterization of purified buffalo beta-lactoglobulin (Buffalo Bulletin (Thailand),35(2))". ไม่ระบุหน่วยงาน. ม.ป.ป.

Siddhartha Narayan Joardar. "Immunochemical characterization of purified buffalo beta-lactoglobulin (Buffalo Bulletin (Thailand),35(2))". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



TARR Wordcloud:
Immunochemical characterization of purified buffalo beta-lactoglobulin (Buffalo Bulletin (Thailand),35(2))
Siddhartha Narayan Joardar
ไม่ระบุผู้เผยแพร่
ไม่ระบุวันที่เผยแพร่
Hydroallantois in a buffalo with fetal anasarca (Buffalo Bulletin (Thailand),35(4)) Endoscopic diagnosis of megaesophagus in a buffalo (Buffalo Bulletin (Thailand),35(2)) Bilateral uncomplicated anopthalmia in a buffalo calf (Buffalo Bulletin (Thailand),35(4)) Dicephalous dipus tetrabrachius calf in a Murrah buffalo (Buffalo Bulletin (Thailand),35(4)) Septicemia in a Murrah buffalo calf: A case report (Buffalo Bulletin (Thailand),35(4)) Ovarian teratoma in a water buffalo (Bubalus bubalis) (Buffalo Bulletin (Thailand),35(4)) Dystocia due to rare foetal monster in a buffalo (Buffalo Bulletin (Thailand),35(4)) Quality characteristics of fermented buffalo beef sausage (Buffalo Bulletin (Thailand),35(4)) Production performance of Nagpuri buffalo under field condition (Buffalo Bulletin (Thailand),35(4)) Management of mummification of fetus in a Murrah buffalo - A case report (Buffalo Bulletin (Thailand),35(4))
คัดลอก URL
กระทู้ของฉัน
ผลการสืบค้นทั้งหมด โพสต์     เรียงลำดับจาก
พัฒนาระบบโดยศูนย์ความรู้เฉพาะด้านวิศวกรรมความรู้และวิศวกรรมภาษาDeveloped by Center of Excellence for Unified Knowledge and Language Engineering
สนับสนุนโดยสำนักงานพัฒนาการวิจัยการเกษตร (องค์การมหาชน)Supported by the Agricultural Research Development Agency (Public Organization)
แบบสำรวจความพึงพอใจQuestionair