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Functional analysis of HbMlo12 in powdery mildew infection and stress response in the rubber tree Hevea brasiliensis Muell Arg.
Yu Zhang - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Functional analysis of HbMlo12 in powdery mildew infection and stress response in the rubber tree Hevea brasiliensis Muell Arg.
ผู้แต่ง / หัวหน้าโครงการ (EN): Yu Zhang
บทคัดย่อ (EN): Powdery mildew locus O (Mlo) gene was first found in barley as a specific powdery mildew resistance gene family. In order to classify the functions of mlo gene in disease resistance and stress response process in rubber tree, a mlo gene, namely Hbmlo12 was cloned from leaves of rubber tree clone CATAS7-33-97. Results showed that the ORF of HbMlo12 was 1721 bp in length, encoding a putative protein of 574 amino acids. HbMlo12 was a typical Mlo protein with eight-transmembrane domain. Sequence comparison between HbMlo12 and other Mlo proteins demonstrated that HbMlo12 shared the highest similarity with the Ricinus communis RcMLO-L12 and Arabidopsis thaliana AtMlo12 with 77% and 64% sequence identity, respectively. Phylogenetic analysis revealed that HbMlo12, RcMlo12-R, RcMlo-L3, AtMlo3, AtMlo12, AtMlo6, and AtMlo2 were grouped into cladeV with 100% bootstrap support value. HbMlo12 expression was tissue specific, and it was preferentially expressed in leaf. HbMlo12 expression significantly changed during powdery mildew infection at the Primary disease. Furthermore, the expression of HbMlo12 was significantly induced by various phytohormones (including ethephon, methyl jasmonate, salicylic acid, abscisic acid, and gibberellic acid). Under drought, wounding stress and indole-3-acetic acid and H2O2 treatment, HbMlo12 expression did not significantly change, but exhibited a complex pattern of regulation. This work provide a theoretical of that HbMlo12 might play a role in phytohormone signaling and abiotic stress response processes in rubber tree.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เผยแพร่โดย (EN): การยางแห่งประเทศไทย
คำสำคัญ (EN): phylogenetic analysis
เจ้าของลิขสิทธิ์ (EN): การยางแห่งประเทศไทย
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Functional analysis of HbMlo12 in powdery mildew infection and stress response in the rubber tree Hevea brasiliensis Muell Arg.
Yu Zhang
การยางแห่งประเทศไทย
ไม่ระบุวันที่เผยแพร่
Multiple Protective roles of the Berberine Bridge Enzyme Gene HbBBE1 in Hevea brasiliensis Muell. Arg. Components of Inorganic Phosphates in Rhizosphere of Rubber Tree (Hevea brasiliensis) De novo transcriptome sequencing of abiotic stress responsive transcripts of Hevea brasiliensis Hydrogen Peroxide is Essential for Wound-Induced Secondary Laticifer Differentiation via Activating Jasmonate Biosynthesis in Rubber Tree (Hevea brasiliensis Müll. Arg.) Studies on Agrobacterium-mediated Genetic Transformation of Embryogenic Cell Suspension and Plant Regeneration in Hevea brasiliensis MicroRNAs: New Regulators of Biological Functions in Hevea brasiliensis CDNA –AFLP ANALYSIS REVEALS DIFFERENTIAL GENE EXPRESSION IN RUBBER TREE (HEVEA BRASILIENSIS) INOCULATED WITH CORYNESPORA CASSIICOLA PATHOGEN Yield Modeling and Analysing the Critical Factors of Productivity of Natural rubber (Hevea brasiliensis) Identifying Pathogenicity Genes in the Rubber Tree Anthracnose Fungus Colletotrichum gloeosporioides through Random Insertional Mutagenesis Expression of NAC Transcription Factor under Drought Stress in Hevea brasiliensis
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