สืบค้นงานวิจัย
Development of pharmabiotics as antibiotic alternatives for seafood security and marine aquaculture health: two cases of study in Vietnam
Van Duy Nguyen - มหาวิทยาลัยขอนแก่น
ชื่อเรื่อง (EN): Development of pharmabiotics as antibiotic alternatives for seafood security and marine aquaculture health: two cases of study in Vietnam
ผู้แต่ง / หัวหน้าโครงการ (EN): Van Duy Nguyen
หน่วยงานสังกัดผู้แต่ง:
  1. Nha Trang University
บทคัดย่อ (EN): Pharmabiotics are any biological materials of gut microbes, including probiotics, bacteriocins, bacteriophages and bioactive molecules. They have been introduced in food, agriculture and clinical settings to replace the use of traditional antibiotics, which led to current threat posed by multi-drug resistant bacteria. This research aims to develop bacteriocins or bacteriocin-producing bacteria as new pharmabiotics for improving seafood security and marine aquaculture health. We screened bacteria isolated from Vietnamese traditional fermented foods and marine animals of interest to the aquaculture industry (lobster, tiger shrimp, otter clam, snubnose pompano and cobia) for antimicrobial and bacteriocin-like activities in order to uncover biodiversity of bacteriocin producers, and explore the potential application in seafood preservation and marine aquaculture. In total, 32 screened isolates showed antimicrobial activities and 15 of these exerted bacteriocin-like activities. Sequencing of 16S rRNA genes identified the isolates as members of the nine genera Lactobacillus, Bacillus, Proteus, Providencia, Klebsiella, Alcaligenes, Enterococcus, Enterobacter and Cronobacter. The bacteriocinogenic isolates showed a wide antimicrobial spectrum against foodborne and animal pathogens, which open the way to their potential use as drugs and probiotics in food, aquaculture, livestock and clinical settings. As the first case of study, two strains Lactobacillus plantarum T8 and T13 were found to produce bacteriocins Class I (Lantibiotics), which remained active at 121°C for 15 min, at pH 4-10 and with proteinase K but deactivated by α-chymotrypsin treatment. The application of culture extract from the strain T13 with cell concentration of 1010 CFU/ml or crude bacteriocin extract from the strain T8 with bacteriocin activity of 800 AU/ml was shown to prolong the chilling preservation of fresh cobia meat compared to control within first 7 days. As the second case of study, the protective effect of bacteriocinogenic Bacillus and Lactobacillus isolates was tested in aquaculture-raised spiny lobster (Panulirus ornatus) juveniles. Lobsters in the probiotic treatments displayed increased growth and reduced feed conversion rates after 60 days, and increased survival rate after pathogen Vibrio owensii DY05 challenge relative to the control. This study represents the first evidence of the use of bacteriocins or bacteriocin producers as biopreservatives for fresh cobia meat and as probiotics for lobsters.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): th
จำนวนหน้า: 9
เอกสารแนบ: https://ag2.kku.ac.th/kaj/PDF.cfm?filename=35-ok.pdf&id=2179&keeptrack=4
เผยแพร่โดย: มหาวิทยาลัยขอนแก่น
คำสำคัญ (EN): probiotics
หากไม่พบเอกสารฉบับเต็ม (Full Text) โปรดติดต่อหน่วยงานเจ้าของข้อมูล

การอ้างอิง

Van Duy Nguyen. "Development of pharmabiotics as antibiotic alternatives for seafood security and marine aquaculture health: two cases of study in Vietnam". มหาวิทยาลัยขอนแก่น. 2557

Van Duy Nguyen. "Development of pharmabiotics as antibiotic alternatives for seafood security and marine aquaculture health: two cases of study in Vietnam". มหาวิทยาลัยขอนแก่น. 2557. Print.



TARR Wordcloud:
Development of pharmabiotics as antibiotic alternatives for seafood security and marine aquaculture health: two cases of study in Vietnam
Van Duy Nguyen
มหาวิทยาลัยขอนแก่น
2557
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