สืบค้นงานวิจัย
Differential Production of Phenylpropanoid Metabolites in Callus Cultures of Ocimum basilicum L. With Distinct in Vitro Antioxidant Activities and in Vivo Protective Effects against UV stress
Nazir M. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Differential Production of Phenylpropanoid Metabolites in Callus Cultures of Ocimum basilicum L. With Distinct in Vitro Antioxidant Activities and in Vivo Protective Effects against UV stress
ผู้แต่ง / หัวหน้าโครงการ (EN): Nazir M.
บทคัดย่อ (EN): Ocimum basilicum L. (Purple basil) is a source of biologically active antioxidant compounds, particularly phenolic acids and anthocyanins. In this study, we have developed a valuable protocol for the establishment of in vitro callus cultures of O. basilicum and culture conditions for the enhanced production of distinct classes of phenylpropanoid metabolites such as hydroxycinnamic acid derivatives (caffeic acid, chicoric acid, rosmarinic acid) and anthocyanins (cyanidin and peonidin). Callus cultures were established by culturing leaf explants on Murashige and Skoog medium augmented with different concentrations of plant growth regulators (PGRs) [thidiazuron (TDZ), α-naphthalene acetic acid (NAA), and 6-benzyl amino purine (BAP)] either alone or in combination with 1.0 mg/L NAA. Among all the above-mentioned PGRs, NAA at 2.5 mg/L led to the highest biomass accumulation (23.2 g/L DW), along with total phenolic (TPP; 210.7 mg/L) and flavonoid (TFP; 196.4 mg/L) production, respectively. HPLC analysis confirmed the differential accumulation of phenolic acid [caffeic acid (44.67 mg/g DW), rosmarinic acid (52.22 mg/g DW), and chicoric acid (43.89 mg/g DW)] and anthocyanins [cyanidin (16.39 mg/g DW) and peonidin (10.77 mg/g DW)] as a function of the PGRs treatment. The highest in vitro antioxidant activity was determined with the ORAC assay as compared to the FRAP assay, suggesting the prominence of the HAT over the ET-based mechanism for the antioxidant action of callus extracts. Furthermore, in vivo results illustrated the protective action of the callus extract to limit the deleterious effects of UV-induced oxidative stress, ROS/RNS production, and membrane integrity in yeast cell culture. Altogether, these results clearly demonstrated the great potential of in vitro callus of O. basilicum as a source of human health-promoting antioxidant phytochemicals. © 2019 American Chemical Society.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-85061484118&doi=10.1021%2facs.jafc.8b05647&partnerID=40&md5=59ebc9dd6871e6893521051df295b1c3
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Ultraviolet Rays
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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Nazir M.. "Differential Production of Phenylpropanoid Metabolites in Callus Cultures of Ocimum basilicum L. With Distinct in Vitro Antioxidant Activities and in Vivo Protective Effects against UV stress". ไม่ระบุหน่วยงาน. ม.ป.ป.

Nazir M.. "Differential Production of Phenylpropanoid Metabolites in Callus Cultures of Ocimum basilicum L. With Distinct in Vitro Antioxidant Activities and in Vivo Protective Effects against UV stress". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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Differential Production of Phenylpropanoid Metabolites in Callus Cultures of Ocimum basilicum L. With Distinct in Vitro Antioxidant Activities and in Vivo Protective Effects against UV stress
Nazir M.
มหาวิทยาลัยมหิดล
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