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Molecular characterization of exon 2-3 of majorhistocompatibility complex (MHC) class I in buffalo (Bubalus bubalis) (Buffalo Bulletin (Thailand),36(3))
Manjit Panigrahi - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Molecular characterization of exon 2-3 of majorhistocompatibility complex (MHC) class I in buffalo (Bubalus bubalis) (Buffalo Bulletin (Thailand),36(3))
ผู้แต่ง / หัวหน้าโครงการ (EN): Manjit Panigrahi
หน่วยงานสังกัดผู้แต่ง (EN): Uttar Pradesh (India). Animal Genetics Division. Animal Genome Laboratory
บทคัดย่อ (EN): Present investigation was undertaken to characterize the exon 2 to 3 of major histocompatibility complex (MHC) class I genes in buffaloes (BuLA-A) of Murrah, Mehsana and Bhadawari breeds using Polymearse chain reaction-restriction length fragment polymorphism (PCR-RFLP). Two primers corresponding to the region of exon 2 intron 2 and exon 3 of BoLA-A were used to amplify the fragment of 714 bp. The PCR-RFLP patterns with respect to DdeI, TaqI, and HinfI restriction enzymes produced distinct polymorphic patterns in all the breeds. Two new patterns with the fragment sizes as 614, 60, 39 bp and 417, 187, 78, 21, 12 bp with DdeI digestion and 608, 97, 9 bp and 609, 106 bp with TaqI digestion were observed. The selected genotypes were cloned and sequenced to find out any change in nucleotides or amino acids with respect to the Holstein Friesian sequence available in the NCBI GenBank. The sequencing results showed that the exon 2- exon 3 products varied from 713 to 715 bp in buffaloes. Polymorphism in exon 2 occurred mostly due to changes of nucleotides at the positions from 62 to 72, 121 to 170 and 184 to 237. The exon 3 initiated at 468 position of nucleotide after intron 2, which had purine rich region conserve sequence CGGGTCA. The phylogenetic tree predicted changes of higher degree in nucleotides as well as amino acids of exon 2- exon 3 and the nucleotide dissimilarity ranged from 3.0 to 14.7%. The results of the present study revealed that the exon 2 to 3 was found polymorhic in the buffaloes.
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ภาษา (EN): en
เอกสารแนบ (EN): https://kukr.lib.ku.ac.th/db/kukr/search_detail/result/371933
คำสำคัญ (EN): India
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การอ้างอิง

Manjit Panigrahi. "Molecular characterization of exon 2-3 of majorhistocompatibility complex (MHC) class I in buffalo (Bubalus bubalis) (Buffalo Bulletin (Thailand),36(3))". ไม่ระบุหน่วยงาน. ม.ป.ป.

Manjit Panigrahi. "Molecular characterization of exon 2-3 of majorhistocompatibility complex (MHC) class I in buffalo (Bubalus bubalis) (Buffalo Bulletin (Thailand),36(3))". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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Molecular characterization of exon 2-3 of majorhistocompatibility complex (MHC) class I in buffalo (Bubalus bubalis) (Buffalo Bulletin (Thailand),36(3))
Manjit Panigrahi
ไม่ระบุผู้เผยแพร่
ไม่ระบุวันที่เผยแพร่
Cryopreservation of buffalo (Bubalus bubalis) semen-limitations and expectations (Buffalo Bulletin (Thailand),36(1)) Congenital anomalies of the uterus in riverine buffalo (Bubalus bubalis) (Buffalo Bulletin (Thailand),36(4)) Clinico-therapeutic management of nervous ketosis in Jaffarabadi buffalo (Bubalus bubalis) (Buffalo Bulletin (Thailand),36(3)) In silico characterization of Mx2 protein of buffalo (Bubalus bubalis) (Buffalo Bulletin,38(4)) Ovarian teratoma in a water buffalo (Bubalus bubalis) (Buffalo Bulletin (Thailand),35(4)) Genetic analysis of major histocompatibility complex (MHC) Class I Exon 4-5 in cattle and buffalo using molecular and phylogenetic approaches (Buffalo Bulletin,40(2)) Screening for genetic disorders in Indian Murrah and Surti buffalo (Bubalus bubalis) bulls (Buffalo Bulletin (Thailand),36(1)) Clinico-biochemical studies of ketosis in buffalo (bubalus bubalis) (Buffalo Bulletin (Thailand),35(1)) Anatomy of the carpal articulation of buffalo calves (Bubalus bubalis) (Buffalo Bulletin (Thailand),35(4)) Oesophageal obstruction in a pregnant buffalo (Buffalo Bulletin (Thailand),36(4))
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