สืบค้นงานวิจัย
Profiling the Proteomic Alterations between the Large and the Small Rubber Particles of Hevea Brasiliensis Using 2D-DIGE
Qiulan Xiang - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Profiling the Proteomic Alterations between the Large and the Small Rubber Particles of Hevea Brasiliensis Using 2D-DIGE
ผู้แต่ง / หัวหน้าโครงการ (EN): Qiulan Xiang
บทคัดย่อ (EN): Natural rubber is synthesized and stored in the rubber particles of Hevea brasiliensis (para rubber tree). It has been demonstrated that the small rubber particles (SRPs) has higher rubber biosynthesis ratio than the large rubber particles (LRPs), but the underlying molecular mechanism still remains unknown. In this study, LRPs and SRPs were firstly separated from the fresh latex using differential centrifugation, and two-dimensional difference in-gel electrophoresis (2D-DiGE) combined with MALDI-TOF/TOF was then applied to investigate the proteomic alterations associated with the changed rubber biosynthesis capacity between LRPs and SRPs. A total of 53 spots corresponding to 22 gene products, were significantly altered with the ratio e”2.0 and T value d’0.05, among which 15 proteins were up-regulated and 7 were down-regulated in the SRPs compared with the LRPs. The 15 up-regulated proteins in the SRPs included small rubber particle protein (SRPP), 3-hydroxy-3-methylglutary1-CoA synthase (HMGCS), phospholipase alpha (PLD ?), ethylene response factor 2, eukaryotic translation initiation factor 5A isoform IV (eIF 5 A-4), 70-kDa heat shock cognate protein (HSC 70), and several unknown proteins etc., whereas the 7 up-regulated proteins in the LRPs were rubber elongation factor (REF, 19.6 kDa). ASR-like protein 1, REF-like stress-related protein 1, a putative phosphoglyceride transfer family protein, ?-1,3-glucanase, a putative retroelement, and a hypothetical protein. The comparative proteome data may provide useful insights into understanding the mechanism involved in rubber biosynthesis and latex coagulation in H. brasiliensis.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เผยแพร่โดย (EN): การยางแห่งประเทศไทย
คำสำคัญ (EN): 2D-DIGE
เจ้าของลิขสิทธิ์ (EN): การยางแห่งประเทศไทย
หากไม่พบเอกสารฉบับเต็ม (Full Text) โปรดติดต่อหน่วยงานเจ้าของข้อมูล

การอ้างอิง


TARR Wordcloud:
Profiling the Proteomic Alterations between the Large and the Small Rubber Particles of Hevea Brasiliensis Using 2D-DIGE
กระทู้ของฉัน
ผลการสืบค้นทั้งหมด โพสต์     เรียงลำดับจาก