สืบค้นงานวิจัย
Antemortem diagnosis of rabies in buffaloes: Molecular approach (Buffalo Bulletin (Thailand),35(4))
Deepti Narang - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Antemortem diagnosis of rabies in buffaloes: Molecular approach (Buffalo Bulletin (Thailand),35(4))
ผู้แต่ง / หัวหน้าโครงการ (EN): Deepti Narang
หน่วยงานสังกัดผู้แต่ง (EN): Punjab (India). Department of Veterinary Microbiology
บทคัดย่อ (EN): Antemortem diagnosis of rabies is highly desirable in animals for limiting the exposure of this fatal zoonotic viral disease. Molecular approaches offer a sensitive tool for detection of rabies viral genome. However, the sensitivity of antemortem detection of rabies from skin of buffalo by molecular approaches has not been compared so far. Comparison of TaqMan real time PCR and heminested reverse transcriptase PCR (HnRTPCR) was, therefore, undertaken to diagnose rabies from skin biopsy samples collected from eleven clinically rabies suspected buffaloes presented to veterinary clinics, GADVASU, Ludhiana, from different districts of Punjab (India). RNA was extracted from all skin samples and converted to cDNA followed by PCR amplification using both molecular methods. Out of 11 cases incorporated in the study 8 skin samples were diagnosed positive using TaqMan real time PCR and HnRTPCR. FAT on brain tissue procured postmortem established same 8 cases as true positive for rabies. Thus, TaqMan real time PCR well as HnRT-PCR detected rabies with 100% sensitivity, specificity and accuracy. The agreement between the results obtained by these methods was 100%, thereby TaqMan real time PCR and HnRT-PCR are equally effective molecular approaches for antemortem diagnosis of rabies from skin biopsies of buffaloes.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://kukr.lib.ku.ac.th/db/kukr/search_detail/result/358466
คำสำคัญ (EN): India
หากไม่พบเอกสารฉบับเต็ม (Full Text) โปรดติดต่อหน่วยงานเจ้าของข้อมูล

การอ้างอิง

Deepti Narang. "Antemortem diagnosis of rabies in buffaloes: Molecular approach (Buffalo Bulletin (Thailand),35(4))". ไม่ระบุหน่วยงาน. ม.ป.ป.

Deepti Narang. "Antemortem diagnosis of rabies in buffaloes: Molecular approach (Buffalo Bulletin (Thailand),35(4))". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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Antemortem diagnosis of rabies in buffaloes: Molecular approach (Buffalo Bulletin (Thailand),35(4))
Deepti Narang
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Diagnosis of rabies in buffaloes: comparison of clinico-pathological, immunohistochemical and immunofluorescent techniques (Buffalo Bulletin (Thailand),35(3)) Endoscopic diagnosis of megaesophagus in a buffalo (Buffalo Bulletin (Thailand),35(2)) Confirmation of rabies in buffalo from organized farm of north Gujarat (Buffalo Bulletin (Thailand),35(4)) Hydroallantois in a buffalo with fetal anasarca (Buffalo Bulletin (Thailand),35(4)) Diagnosis of Dermatophilus dermatitis among buffaloes in Kerala (Buffalo Bulletin (Thailand),35(1)) Bilateral uncomplicated anopthalmia in a buffalo calf (Buffalo Bulletin (Thailand),35(4)) Ovarian teratoma in a water buffalo (Bubalus bubalis) (Buffalo Bulletin (Thailand),35(4)) Dystocia due to rare foetal monster in a buffalo (Buffalo Bulletin (Thailand),35(4)) Quality characteristics of fermented buffalo beef sausage (Buffalo Bulletin (Thailand),35(4)) Production performance of Nagpuri buffalo under field condition (Buffalo Bulletin (Thailand),35(4))
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