คลังข้อมูลการวิจัยการเกษตรไทย

ผู้แต่ง Ruchusatsawat

เผยแพร่โดย

1 National Institute of Health, Department of Medical Sciences, Nontaburi 11000, Thailand 2 Center of Excellence in Molecular Genetics of Cancer and Human Diseases, Department of Anatomy, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand 3 Center of Excellence in Immunology and Immune Mediated Diseases, Division of Immunology, Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand

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งานวิจัยที่แนะนำ Cloning and expression of HbACO2 of Hevea brasiliensis ตอนที่ 31 : รางวัลนักวิจัยดีเด่นระดับนานาชาติ และนักวิจัยดีเด่นระดับชาติ Cloning and expression of 3-hydroxy-3-methylglutaryl-Coa Reductase 1 (Hmge 1) from Hevea brasiliensis Functional characterization of metallothionein-like genes from Physcomitrella patens: expression profiling, yeast heterologous expression, and disruption of PpMT1.2a gene Gene expression and promoter characterization of heat-shock protein 90B gene (HSP90B) in the model unicellular green alga Chlamydomonas reinhardtii Trichoharzianol, a New Antifungal from Trichoderma harzianum F031 Expression of NAC Transcription Factor under Drought Stress in Hevea brasiliensis Expression and characterization of glutathione peroxidase of the liver fluke, Fasciola gigantica AQUAPORIN GENES EXPRESSION IN THE TRUNK PHLOEM AND IN THE LATICIFERS OF RUBBER TREE Effect of iron overload on furin expression in wild-type and β-thalassemic mice

คัดลอก URL

ชื่อเรื่อง (EN):	BCAP 31 expression and promoter demethylation in psoriasis
ผู้แต่ง / หัวหน้าโครงการ (EN):	Ruchusatsawat
บทคัดย่อ (EN):	Background: Psoriasis is the disease of abnormal keratinocyte differentiation and apoptosis. Alterations in DNA methylation leading to keratinocyte hyperproliferation is one of the proposed pathogenic mechanisms of psoriasis. B-cell receptor associated protein (BCAP31) has been reported to be involved in the proliferation and apoptosis of keratinocytes. Up-regulation and changing in BCAP31 promoter methylation has been reported to be associated with some cancers. To date, there has been no study of psoriasis. Objective: We investigated BCAP31 protein expression and the status of BCAP31 promoter methylation in psoriasis. Methods: Ten patients with psoriasis and 10 healthy subjects were enrolled. The immunohistochemistry was performed on paraffin-embedded tissue section to detect BCAP31 protein expression and compared between psoriasis and normal skin. The laser capture micro-dissected keratinocyte were analyzed using bisulfite PCR method and cloning and sequencing. Results: Increased BCAP31 protein expression was observed in psoriatic epidermis compared with normal epidermis. Interestingly the methylation level of the BCAP31 promoter was significantly lower in patients with psoriasis compared with healthy subjects (p < 0.001, % psoriasis vs. normal skin methylation = 14.94 vs. 60.61). Conclusions: The present study demonstrated increase expression of BCAP31 protein related to BCAP31 DNA demethylation in psoriasis. Future study is needed to indicate the mechanism of BCAP31 promoter demethylation and its potential use as a novel treatment for psoriasis in the future.
บทคัดย่อ:	ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN):	en
เอกสารแนบ (EN):	http://nih.dmsc.moph.go.th/research/showimgdetil.php?id=509
เผยแพร่โดย (EN):	1 National Institute of Health, Department of Medical Sciences, Nontaburi 11000, Thailand 2 Center of Excellence in Molecular Genetics of Cancer and Human Diseases, Department of Anatomy, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand 3 Center of Excellence in Immunology and Immune Mediated Diseases, Division of Immunology, Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
คำสำคัญ (EN):	Promoter methylation; Protein expression; Immunohistochemistry; BCAP31 and psoriasis
เจ้าของลิขสิทธิ์ (EN):	National Institute of Health, Department of Medical Science