สืบค้นงานวิจัย
Preparation of a highly specific single chain variable fragment antibody targeting miroestrol and its application in quality control of Pueraria candollei by enzyme-linked immunosorbent assay
Pongkitwitoon B. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Preparation of a highly specific single chain variable fragment antibody targeting miroestrol and its application in quality control of Pueraria candollei by enzyme-linked immunosorbent assay
ผู้แต่ง / หัวหน้าโครงการ (EN): Pongkitwitoon B.
บทคัดย่อ (EN): Introduction: Miroestrol is the potent phytoestrogen isolated from White Kwao Krua (Pueraria candollei var. mirifica (Airy Shaw & Suvat.) Niyomdham, a Thai traditional medicinal plant. Nowadays, various health supplementary products featuring White Kwao Krua are available worldwide. A sensitive and rapid analytical method for quantification of miroestrol is necessary for quality control of these products. Objectives: To prepare a single-chain variable fragment (scFv) antibody specific to miroestrol and develop a scFv-based enzyme-linked immunosorbent assay (ELISA) for quantitative analysis of miroestrol in plant materials and health supplementary products. Methods: A gene encoding anti-miroestrol scFv antibody was constructed and expressed in Escherichia coli SHuffle T7 strain. Anti-miroestrol scFv antibody was characterised and applied to ELISA. The developed scFv-based ELISA method was validated for its sensitivity, specificity, accuracy and precision. Results: Anti-miroestrol scFv antibody was highly specific to miroestrol. The scFv-based ELISA was applied to determine miroestrol in the range 0.06–7.81 μg/mL, with the limit of quantification of 0.06 μg/mL miroestrol. The accuracy of the assay was validated by its 95.08–103.99% recovery from the spiked miroestrol recovery experiment and in good correlation with the results from the monoclonal antibody-based ELISA. The relative standard deviation of the intra- and inter-assay were less than 6.0%. Conclusion: The developed scFv-based ELISA was sensitive, specific, accurate, and precise for determination of miroestrol and useful for quality control of P. candollei plant raw materials and supplementary products. © 2019 John Wiley & Sons, Ltd.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-85065016571&doi=10.1002%2fpca.2832&partnerID=40&md5=cb611914d654ccb00801d51b2342f200
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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การอ้างอิง

Pongkitwitoon B.. "Preparation of a highly specific single chain variable fragment antibody targeting miroestrol and its application in quality control of Pueraria candollei by enzyme-linked immunosorbent assay". ไม่ระบุหน่วยงาน. ม.ป.ป.

Pongkitwitoon B.. "Preparation of a highly specific single chain variable fragment antibody targeting miroestrol and its application in quality control of Pueraria candollei by enzyme-linked immunosorbent assay". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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Preparation of a highly specific single chain variable fragment antibody targeting miroestrol and its application in quality control of Pueraria candollei by enzyme-linked immunosorbent assay
Pongkitwitoon B.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
การตรวจสอบสารพิษแอฟลาทอกซิน โดยวิธี ELISA (Enzyme - Linked Immunosorbent Assay) QUALITY CONTROL IN LONGAN PROCESSING Rhizobium puerariae sp. nov., an endophytic bacterium from the root nodules of the medicinal plant Pueraria candollei var. candollei Factors involved in Natural rubber Consistency linked to the antivibration application Development of botulinum toxin detection assay by antibody capture ELISA ScFv Antibody Specific to Cassiicolin, a Host-Selective Toxin of Corynespora cassiicola Detection of runs of homozygosity in Norwegian Red: Density, criteria and genotyping quality control Motion control applications: Observer based DC motor parameters estimation for novices Influenza neuraminidase subtype N1: Immunobiological properties and functional assays for specific antibody response Simultaneous detection of feces-specific bacteriophages of bacteroides fragilis with a duplex PCR assay
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