สืบค้นงานวิจัย
Presence of Penaeus monodon densovirus in the ovary of chronically infected P. monodon subadults
Fernando S. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Presence of Penaeus monodon densovirus in the ovary of chronically infected P. monodon subadults
ผู้แต่ง / หัวหน้าโครงการ (EN): Fernando S.
บทคัดย่อ (EN): Shrimp infected with Penaeus monodon densovirus (PmoDNV) usually display no specific gross signs, but heavy infections can kill postlarvae and retard juvenile growth. In the present study, samples of hepatopancreas, feces, gonads and hemolymph were isolated from male and female P. monodon subadults chronically infected by PmoDNV. Each sample of hepatopancreas and gonad was divided into 2 parts: one for PmoDNV detection by polymerase chain reaction (PCR), and the other for routine histology and immunohistochemistry. The frequency of positive findings via PCR assays was 92% in the hepatopancreas, 57% in feces, 50% in ovary, 35% in hemolymph and 0% in the testis. Using the densitometric value (DV) of the specific band for PmoDNV relative to that of the β-actin gene as an index of the viral load in the samples, no significant differences were observed among sample types and sexes. Hematoxylin-eosin staining of infected hepatopancreas revealed typical PmoDNV inclusions in the nuclei of infected cells. The ovaries with high DV (>1) contained various types of inclusions along the row of the follicular cells or possibly in the connective tissue cells surrounding the oocytes. Using immunohistochemistry with specific probes to detect PmoDNV proteins, a positive reaction was observed in viral inclusions found in infected hepatopancreas and in ovaries with high DV, specifically in the ovarian capsule, hemolymph, oocytes and nuclear inclusions. These results suggest that the localization of PmoDNV in P. monodon is not confined to the hepatopancreas, but rather that the virus can also occur in the ovary; hence, trans-ovarian, vertical transmission of the virus is highly possible. © Inter-Research 2018.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-85052145244&doi=10.3354%2fdao03241&partnerID=40&md5=60c9131c022f160841b69943182a438c
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Penaeus monodon
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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Fernando S.. "Presence of Penaeus monodon densovirus in the ovary of chronically infected P. monodon subadults". ไม่ระบุหน่วยงาน. ม.ป.ป.

Fernando S.. "Presence of Penaeus monodon densovirus in the ovary of chronically infected P. monodon subadults". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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Presence of Penaeus monodon densovirus in the ovary of chronically infected P. monodon subadults
Fernando S.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
Needle biopsy of the hepatopancreas of the black tiger shrimp Penaeus monodon with Penaeus monodon densovirus detection An evidence on trans-ovarian transmission of Monodon baculovirus (MBV) infection in Penaeus monodon Identification and expression of white spot syndrome virus-encoded microRNAs in infected Penaeus monodon Yellow head virus binding to cell surface proteins from Penaeus monodon hemocytes Rab5, an early endosomal protein required for yellow head virus infection of Penaeus monodon Australian red claw crayfish (Cherax quadricarinatus) is susceptible to yellow head virus (YHV) infection and can transmit it to the black tiger shrimp (Penaeus monodon) Evidence that the whiteleg shrimp Penaeus (Litopenaeus) vannamei is refractory to infection by Penaeus monodon nudivirus (PmNV), also known as MBV Alteration of egg activation by protease inhibitors in the black tiger shrimp, Penaeus monodon Correlation between gonad-inhibiting hormone and vitellogenin during ovarian maturation in the domesticated Penaeus monodon Successful yellow head virus infection of Penaeus monodon requires clathrin heavy chain
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