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Interaction study of a novel Macrobrachium rosenbergii effector caspase with B2 and capsid proteins of M.rosenbergii nodavirus reveals their roles in apoptosis
Youngcharoen S. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Interaction study of a novel Macrobrachium rosenbergii effector caspase with B2 and capsid proteins of M.rosenbergii nodavirus reveals their roles in apoptosis
ผู้แต่ง / หัวหน้าโครงการ (EN): Youngcharoen S.
บทคัดย่อ (EN): Apoptosis is an essential immune response to protect invertebrates from virus infected cells. In shrimp, virus infection has been reported to induce apoptosis. Macrobrachium rosenbergii (. Mr) was considered to be a disease-resistant host when compared to penaeid shrimps. Caspase-3 was classified as an executioner caspase which played a key role in virus-induced apoptosis. In this study, an effector caspase gene of M.rosenbergii (. Mrcasp) was cloned and characterized. The open reading frame (ORF) of Mrcasp was 957 nucleotide encoding 318 amino acid with a deduced molecular mass of 35.87kDa. RT-PCR analysis showed the presence of Mrcasp in all examined tissues. The phylogenetic tree indicated that Mrcasp was closely related with caspase 3 of shrimp. The functions of the Mrcasp, B2 and capsid proteins of M. rosenbergii nodavirus (. MrNV) were assayed in Sf-9 cells. The results showed that Mrcasp induce apoptotic morphology cells; however, capsid protein of MrNV could inhibit apoptotic cells whereas B2 could neither induce nor inhibit apoptotic cells by DAPI staining. The protein interaction between Mrcasp and viral MrNV structure revealed that Mrcasp did not bind to B2 or capsid protein whereas B2 and capsid proteins could bind directly to each other. This study reported a novel sequence of a full-length Mrcasp and its functional studies indicated that Mrcasp could induce apoptotic cells. Our data is the first report demonstrating the direct protein-protein interaction between capsid protein and B2 protein of MrNV. © 2015 Elsevier Ltd.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-84930204530&doi=10.1016%2fj.fsi.2015.05.009&partnerID=40&md5=bad18c788682dfebab3f5641c52f85af
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Viral Proteins
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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Interaction study of a novel Macrobrachium rosenbergii effector caspase with B2 and capsid proteins of M.rosenbergii nodavirus reveals their roles in apoptosis
Youngcharoen S.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
The existence of kisspeptin-like peptides and effects on ovarian development and maturation in the giant freshwater prawn Macrobrachium rosenbergii Hematopoietic tissue of Macrobrachium rosenbergii plays dual roles as a source of hemocyte hematopoiesis and as a defensive mechanism against Macrobrachium rosenbergii nodavirus infection Distinctive histopathology of Spiroplasma eriocheiris infection in the giant river prawn Macrobrachium rosenbergii Vitellogenin gene expression at different ovarian stages in the giant freshwater prawn, Macrobrachium rosenbergii, and stimulation by 4-nonylphenol ผลของสไปรูไลนา (Spirulina platensis) ต่ออัตราการรอดของกุ้งก้ามกราม (Macrobrachium rosenbergii ) ที่ติดเชื้อ Macrobrachium rosenbergii Nodavirus และ Extra small virus Delivery of double stranded RNA by Macrobrachium rosenbergii nodavirus-like particles to protect shrimp from white spot syndrome virus Identification and characterization of a crustacean female sex hormone in the giant freshwater prawn, Macrobrachium rosenbergii The interaction between PmHtrA2 and PmIAP and its effect on the activity of Pm caspase Molecular isolation and characterization of a spätzle gene from Macrobrachium rosenbergii Molecular cloning and characterization of a Toll receptor gene from Macrobrachium rosenbergii
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