คลังข้อมูลการวิจัยการเกษตรไทย

ผู้แต่ง Sarawut Toonkomdang

เผยแพร่โดย

1 Department of Medical Technology, Maesot General Hospital, Tak, Thailand 2 Department of Family Medicine, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand 3 Center for Clinical Epidemiology and Clinical Statistics, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand 4 Department of Medical Sciences, National Institute of Health, Ministry of Public Health, Nonthaburi, Thailand

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งานวิจัยที่แนะนำ Development LAMP technique for detecting leptospirosis at Thai NIH การพัฒนาวิธีการตรวจโรคเมลิออยโดสิสและเลปโตสไปโรสิสด้วย loop-mediated isothermal amplification (LAMP) Development of loop-mediated isothermal amplification (LAMP) for diagnosing melioidosis and leptosp Thai Cosmetopoeia การขับเคลื่อนเศรษฐกิจสังคมไทยด้วยการสร้างสรรคุณค่าเครื่องสำอางตามเอกลักษณ์ท้องถิ่น Comparison of Loop-Mediated Isothermal Amplification, Microscopy, Culture, and PCR for Diagnosis of Pulmonary Tuberculosis T‑SPOT®.TB Test for Latent Tuberculosis infection Diagnosis and Treatment Guidance in Thai Health‑Care Professionals Thai Open GLAM การพัฒนาวิธีการตรวจโรคเมลิออยโดสิสด้วย loop-mediated isothermal amplification (LAMP) Development of loop-mediated isothermal amplification (LAMP) for diagnosing melioidosis การพัฒนาและเพิ่มผลผลิตปลาสวายโมง (Thai Panga) เพื่อการส่งออก Genotyping of Mycobacterium tuberculosis by Spoligotyping การตรวจแยกสายพันธุ์เชื้อวัณโรคด้วยวิธี Spoligotyping Use of dried blood spot specimens for in-house HIV drug resistance genotyping assay among infants in Thailand

คัดลอก URL

ชื่อเรื่อง (EN):	Pragmatic accuracy of an in-house loop-mediated isothermal amplification (LAMP) for diagnosis of pulmonary tuberculosis in a Thai community hospital
ผู้แต่ง / หัวหน้าโครงการ (EN):	Sarawut Toonkomdang
บทคัดย่อ (EN):	Background: To improve the quality of diagnosing pulmonary tuberculosis (TB), WHO recommends the use of rapid molecular testing as an alternative to conventional microscopic methods. Loop-mediated isothermal amplification assay (LAMP test) is a practical and cost-effective nucleic amplification technique. We evaluated the pragmatic accuracy of an in-house LAMP assay for the diagnosis of TB in a remote health care setting where an advanced rapid molecular test is not available. Methods: A prospective diagnostic accuracy study was conducted. Patients with clinical symptoms suggestive of TB were consecutively enrolled from April to August 2016. Sputum samples were collected from each patient and were sent for microscopic examination (both acid-fast stain and fluorescence stain), in-house LAMP test, and TB culture. Results: One hundred and seven patients with TB symptoms were used in the final analysis. This included 50 (46.7%) culture-positive TB patients and 57 (53.3%) culture-negative patients. The overall sensitivity of the in-house LAMP based on culture positivity was 88.8% (95/107) with a 95%CI of 81.2-94.1. The sensitivity was 90.9% (40/44) with a 95%CI of 78.3-97.5 for smear-positive, culture-positive patients, and was 16.7% (1/6) with a 95%CI of 0.4-64.1 for smear-negative, culture-positive patients. The overall sensitivity of the in-house LAMP test compared to smear microscopy methods were not significantly different (p = 0.375). The specificity of the in-house LAMP based on non-TB patients (smear-negative, culture-negative) was 94.7% (54/57) with a 95%CI of 85.4-98.9. Conclusions: The diagnostic accuracy of the in-house LAMP test in a community hospital was comparable to other previous reports in terms of specificity. The sensitivity of the in-house assay could be improved with better sputum processing and DNA extraction method
บทคัดย่อ:	ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN):	en
เอกสารแนบ (EN):	http://nih.dmsc.moph.go.th/research/showimgdetil.php?id=579
เผยแพร่โดย (EN):	1 Department of Medical Technology, Maesot General Hospital, Tak, Thailand 2 Department of Family Medicine, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand 3 Center for Clinical Epidemiology and Clinical Statistics, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand 4 Department of Medical Sciences, National Institute of Health, Ministry of Public Health, Nonthaburi, Thailand
คำสำคัญ (EN):	Thai community hospital
เจ้าของลิขสิทธิ์ (EN):	National Institute of Health, Department of Medical Science