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Enzyme matching design approach on very high gravity liquefaction and saccharification of cassava root and cassava starch for ethanol fermentation
Sakdaronnarong C. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Enzyme matching design approach on very high gravity liquefaction and saccharification of cassava root and cassava starch for ethanol fermentation
ผู้แต่ง / หัวหน้าโครงการ (EN): Sakdaronnarong C.
บทคัดย่อ (EN): During ethanol production, the ratio of various sources of starch-hydrolyzing enzymes significantly influences hydrolysis efficiency in a very high gravity (VHG) system. In this study, the enzyme matching approach was proposed to optimize liquefaction and saccharification yield of cassava root and cassava starch. For fresh cassava root, the synergistic effect of using Techzyme Q-Add enzyme (93 °C pH 5.6) for liquefaction and GC 147 enzyme (61.5 °C pH 4.2) for saccharification achieved the highest yield among all enzyme-matching designs. At 25% solid, highest total reducing sugar (TRS) yields from sequential liquefaction and saccharification of cassava starch, fresh cassava, and dry cassava were 87.9%, 85.1%, and 70.0% corresponding to 286.8, 249.4, and 241.1 g/L, respectively. For VHG ethanol fermentation by Saccharomyces cerevisiae TISTR 5606, separate liquefaction and simultaneous saccharification and fermentation (SLSSF) gave a significantly higher ethanol concentration compared with separate liquefaction and saccharification and fermentation (SLSF). From a 35% solid SLSSF system, the highest ethanol produced at 30 °C was 27.3 g/L from 72 h, respectively. The results suggested that SLSSF could effectively shorten the time course of the whole process for liquefaction, saccharification, and fermentation from 74 h to only 26 h for similar ethanol production yields. © 2018 North Carolina State University.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-85045188722&doi=10.15376%2fbiores.13.2.2304-2327&partnerID=40&md5=2aabb7ef4f0c22cddbb46e3f3934526d
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Starch
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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Enzyme matching design approach on very high gravity liquefaction and saccharification of cassava root and cassava starch for ethanol fermentation
Sakdaronnarong C.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
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