สืบค้นงานวิจัย
Optimisation and standardisation of functional immune assays for striped catfish (Pangasianodon hypophthalmus) to compare their immune response to live and heat killed Aeromonas hydrophila as models of infection and vaccination
Sirimanapong W. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Optimisation and standardisation of functional immune assays for striped catfish (Pangasianodon hypophthalmus) to compare their immune response to live and heat killed Aeromonas hydrophila as models of infection and vaccination
ผู้แต่ง / หัวหน้าโครงการ (EN): Sirimanapong W.
บทคัดย่อ (EN): Aquaculture production of Pangasianodon hypophthalmus is growing rapidly in South East Asia, especially in Vietnam. As it is a relatively new aquaculture species there are few reports evaluating its immune response to pathogens. Thus, functional assays for P.hypophthalmus were optimised to evaluate both innate and adaptive immune responses, and were then used to examine immune response following stimulation with live and heat-killed Aeromonas hydrophila. These were used as models of infection and vaccination, respectively.Four treatment groups were used, including a control group, a group injected intraperitonally (IP) with adjuvant only, a group injected with heat-killed A.hydrophila (1×109cfuml-1 mixed with adjuvant), and a group injected with a subclinical dose of live A.hydrophila. Samples were collected at 0, 1, 3, 7, 14 and 21 days post-injection (d.p.i.) to assess their immune response. The results indicated that challenge with live or dead bacteria stimulated the immune response in P.hypophthalmus significantly above the levels observed in control groups with respect to specific antibody titre, plasma lysozyme and peroxidase activity, and phagocytosis by head kidney macrophages at 7 or/and 14d.p.i. At 21d.p.i., total and specific antibody (IgM) levels and plasma lysozyme activity in fish injected with either live or dead A.hydrophila were significantly different to the control groups. Differential immune responses were observed between fish injected with either live or dead bacteria, with live A.hydrophila significantly stimulating an increase in WBC counts and plasma peroxidase activity at 3d.p.i., with the greatest increase in WBC counts noted at 21d.p.i. and in phagocytosis at 14d.p.i. By 21d.p.i. only the macrophages from fish injected with dead A.hydrophila showed significantly stimulation in their respiratory burst activity. This study provides basic information on the immune response in pangasius catfish that can be useful in the health control of this species. © 2014 Elsevier Ltd.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-84905915104&doi=10.1016%2fj.fsi.2014.07.021&partnerID=40&md5=5ab22843ea9042fef891740e40e40886
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Vaccination
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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Sirimanapong W.. "Optimisation and standardisation of functional immune assays for striped catfish (Pangasianodon hypophthalmus) to compare their immune response to live and heat killed Aeromonas hydrophila as models of infection and vaccination". ไม่ระบุหน่วยงาน. ม.ป.ป.

Sirimanapong W.. "Optimisation and standardisation of functional immune assays for striped catfish (Pangasianodon hypophthalmus) to compare their immune response to live and heat killed Aeromonas hydrophila as models of infection and vaccination". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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Optimisation and standardisation of functional immune assays for striped catfish (Pangasianodon hypophthalmus) to compare their immune response to live and heat killed Aeromonas hydrophila as models of infection and vaccination
Sirimanapong W.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
The effects of feeding immunostimulant β-glucan on the immune response of Pangasianodon hypophthalmus Concurrent infections of Flavobacterium columnare and Edwardsiella ictaluri in striped catfish, Pangasianodon hypophthalmus in Thailand The effects of feeding β-glucan to Pangasianodon hypophthalmus on immune gene expression and resistance to Edwardsiella ictaluri Francisella noatunensis subsp. orientalis infects striped catfish (Pangasianodon hypophthalmus) and common carp (Cyprinus carpio) but does not kill the hosts Immune response to recombinant Burkholderia pseudomallei FliC Insights into the naturally acquired immune response to Plasmodium vivax malaria Naturally-acquired immune response against Plasmodium vivax rhoptry-associated membrane antigen In vitro production of functional immune cells derived from human hematopoietic stem cells Influenza neuraminidase subtype N1: Immunobiological properties and functional assays for specific antibody response Epr3 is a conserved immunogenic protein among Aeromonas species and able to induce antibody response in Nile tilapia
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