สืบค้นงานวิจัย
TECHNIQUES FOR STUDYING THE TRANSPORTATION OF JASMONIC ACID IN HEVEA BRANSILINSIS
Shi Mingjing - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): TECHNIQUES FOR STUDYING THE TRANSPORTATION OF JASMONIC ACID IN HEVEA BRANSILINSIS
ผู้แต่ง / หัวหน้าโครงการ (EN): Shi Mingjing
บทคัดย่อ (EN): An attempt was made to develop techniques of autoradiography in paraffin section and indirect immunofluorescence localization to explore the transportation of exogenous jasmonic acid in the rubber tree (Hevea brasiliensis Mull.Arg). Jasmonic acid is and important and novel plant hormone. At present, transportation of jasmonic acid in plant was rarely studied for the difficulty in fixing jasmonic acid. Some reagents were found to resolve the problem in investigation the transportation of jasmonic acid in rubber tree. By using autoradiography in paraffin section, the distribution of 3H-JA was identified. The 3H-JA was observed in phloem and xylem at the young stems where 3H-JA was applied and its adjacent positions. The results suggested that JA could be transported rapidly from phloem to xylem through cambium and transported upwards as well as downwards through vessel tube and sieve tube. Immunofluorescence localization showed that exogenous JA was distributed mainly in the phloem during 30 minutes to 1 day after JA application. The results were testified with works made by other researchers using radioisotope technique. However, our techniques can mark the idiographic location of JA in the rubber tree, but radioisotope technique does not. The techniques of autoradiography in paraffin section and indirect Immunofluorescence localization provided feasible approaches for studying the transportation of JA in the rubber tree and further afforded reference for investigating the physiological function of JA in the rubber tree.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เผยแพร่โดย (EN): การยางแห่งประเทศไทย
คำสำคัญ (EN): indirect immunfluorescence localization.
เจ้าของลิขสิทธิ์ (EN): การยางแห่งประเทศไทย
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