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Lysinibacillus sphaericus binary toxin induces apoptosis in susceptible Culex quinquefasciatus larvae
Tangsongcharoen C. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Lysinibacillus sphaericus binary toxin induces apoptosis in susceptible Culex quinquefasciatus larvae
ผู้แต่ง / หัวหน้าโครงการ (EN): Tangsongcharoen C.
บทคัดย่อ (EN): During sporulation, a Gram-positive bacterium Lysinibacillus sphaericus (. Ls) produces the mosquito larvicidal binary toxin composed of 2 subunits, BinA and BinB. Full toxicity against Culex and Anopheles mosquito larvae is achieved when both subunits are administered together at equimolar amounts. Although cellular responses to Bin toxin have been reported in previous studies, it remains essential to extensively examine the cytopathic effects in vivo to define the underlying mechanism of larval death. In this study, 4th instar Culex quinquefasciatus larvae fed with different doses of Bin toxin were analyzed both for ultrastructural as well as biochemical effects. Typical morphological changes consistent with apoptosis were observed in mosquito larvae exposed to Bin toxin, including mitochondrial swelling, chromatin condensation, cytoplasmic vacuolization and apoptotic cell formation. Bin toxin also induced the activation of caspase-9 and caspase-3 in larval midgut cells. Our current observations thus suggest that Bin toxin triggers apoptosis via an intrinsic or mitochondrial pathway in vivo, possibly contributing to larval death. © 2015 Elsevier Inc.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-84929468594&doi=10.1016%2fj.jip.2015.04.008&partnerID=40&md5=367b514304ddfef39406999d7a68c7b2
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Microscopy, Electron, Transmission
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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Lysinibacillus sphaericus binary toxin induces apoptosis in susceptible Culex quinquefasciatus larvae
Tangsongcharoen C.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
Molecular analysis of Culex quinquefasciatus larvae responses to Lysinibacillus sphaericus bin toxin Suppression of α-glucosidase gene by RNA interference can increase resistance of Culex quinquefasciatus to Bacillus sphaericus binary toxin Interaction of Lysinibacillus sphaericus binary toxin with mosquito larval gut cells: Binding and internalization Binary toxin subunits of lysinibacillus sphaericus are monomeric and form heterodimers after in vitro activation Development of B-Soy starter for biolarvicide production for control of Culex quinquefasciatus mosquito larvae Efficiency of Tinospora crispa against Culex quinquefasciatus larva Excretory-secretory product of third-stage Gnathostoma spinigerum larvae induces apoptosis in human peripheral blood mononuclear cells Monitoring of resistance in laboratory susceptible strain and field population strains of Culex quinquefasciatus to Bacillus thuringiensis var. israelensis (Bti), Bacillus sphaericus (Bsph) and temeph Development of larvicidal Bacillus thuringiensis var. israelensis by the Thai NIH and its comparison to Bacillus sphaericus and temephos in a selection experiment with the mosquito Culex quinquefascia Biological Control of Culex spp. Larvae by Bacillus thuringiensis subsp. israelensis Cultured From Different Local Waste Materials
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