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Multiplex-PCR As a rapid and sensitive method for identification of meat species in halal-meat products
Alikord M. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Multiplex-PCR As a rapid and sensitive method for identification of meat species in halal-meat products
ผู้แต่ง / หัวหน้าโครงการ (EN): Alikord M.
บทคัดย่อ (EN): Species identification and authentication in meat products are important subjects for ensuring the health of consumers. The multiplex-PCR amplification and species- specific primer set were used for the identification of horse, donkey, pig and other ruminants in raw and processed meat products. Methods: Oligonucleotid primers were designed and patented for amplification of species-specific mitochondrial DNA sequences of each species and samples were prepared from binary meat mixtures. Results: The results showed that meat species were accurately determined in all combinations by multiplex-PCR, and the sensitivity of this method was 0.001 ng, rendering this technique open to and suitable for use in industrial meat products. It is concluded that more fraud is seen in lower percentage industrial meat products than in higher percentage ones. There was also more fraud found in processed products than in raw ones. Conclusion: This rapid and useful test is recommended for quality control firms for applying more rigorous controls over industrial meat products, for the benefit of target consumers. © 2016 Bentham Science Publishers.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-85030311187&doi=10.2174%2f2212798409666170113151213&partnerID=40&md5=d00f536868c762d889a19dbc0cfcd7b9
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Swine
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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Multiplex-PCR As a rapid and sensitive method for identification of meat species in halal-meat products
Alikord M.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
Rapid detection of Campylobacter jejuni, Campylobacter coli, and Campylobacter lari in fresh chicken meat and by-products in Bangkok, Thailand, using modified multiplex PCR A rapid and sensitive spectrophotometric method for the determination of benzoyl peroxide in wheat flour samples Meat quality assessment of local cattle and buffalo through nutritive and physiochemical evaluation of blood and meat (Buffalo Bulletin,40(3)) Identification of veillonella species in the tongue biofilm by using a novel one-step polymerase chain reaction method PLANT-BASED-MEAT (เนื้อจากพืช) Multi-probe Real-Time PCR Identification of Four Common Candida Species in Blood Culture Broth Development of laboratory test for differentiation of Dengue, Chikungunya and Zika viruses by multiplex real-time RT-PCR method Single nucleotide polymorphism-based multiplex PCR for identification and genotyping of the oomycete Pythium insidiosum from humans, animals and the environment Diversity analysis of Theileria species in local buffaloes of Odisha, India (Buffalo Bulletin,39(4)) Rapid Molecular Identification and Detection of Corynespora cassiicola of Hevea brasiliensis
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