สืบค้นงานวิจัย
Immunodiagnosis of fasciola gigantica infection using monoclonal antibody-based sandwich ELISA and Immunochromatographic Assay for detection of circulating cathepsin L1 Protease
Anuracpreeda P. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Immunodiagnosis of fasciola gigantica infection using monoclonal antibody-based sandwich ELISA and Immunochromatographic Assay for detection of circulating cathepsin L1 Protease
ผู้แต่ง / หัวหน้าโครงการ (EN): Anuracpreeda P.
บทคัดย่อ (EN): Tropical fasciolosis caused by Fasciola gigantica infection is one of the major diseases infecting ruminants in the tropical regions of Africa and Asia including Thailand. Parasitological diagnosis of fasciolosis is often unreliable and possesses low sensitivity. Therefore, the detection of circulating parasite antigens is thought to be a better alternative for diagnosis of fasciolosis, as it reflects the real parasite burden. Methods In this study, we have produced a monoclonal antibody (MoAb) against recombinant F. gigantica cathepsin L1 (rFgCatL1), and developed both sandwich enzyme-linked immunosorbent assay (sandwich ELISA) and immunochromatographic (IC) test for rapid detection of circulating cathepsin L1 protease (CatL1) in the sera from mice experimentally and cattle naturally infected with Fasciola gigantica. MoAb 4E3 and biotinylated rabbit anti-recombinant CatL1 antibody were selected due to their high reactivities and specificities. Results The lower detection limits of sandwich ELISA and IC test were 3 pg/ml and 0.256 ng/ml, respectively. Sandwich ELISA and IC test could detect F. gigantica infection from day 1 to 35 post infection. In experimental mice, the sensitivity, specificity and accuracy were 95%, 100% and 98.6% (for sandwich ELISA), and 93%, 100% and 98.2% (for IC test), while in natural cattle they were 98.3%, 100% and 99.5%(for sandwich ELISA), and 96.7%, 100% and 99.1% (for IC test). Conclusions These two assay methods showed high efficiencies and precisions for diagnosis of fasciolosis by F. gigantica. © 2016 Anuracpreeda et al.This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-84953790549&doi=10.1371%2fjournal.pone.0145650&partnerID=40&md5=b767949c6c27d7505a3e0e01056eafd7
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): helminth protein
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
หากไม่พบเอกสารฉบับเต็ม (Full Text) โปรดติดต่อหน่วยงานเจ้าของข้อมูล

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Anuracpreeda P.. "Immunodiagnosis of fasciola gigantica infection using monoclonal antibody-based sandwich ELISA and Immunochromatographic Assay for detection of circulating cathepsin L1 Protease". ไม่ระบุหน่วยงาน. ม.ป.ป.

Anuracpreeda P.. "Immunodiagnosis of fasciola gigantica infection using monoclonal antibody-based sandwich ELISA and Immunochromatographic Assay for detection of circulating cathepsin L1 Protease". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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Immunodiagnosis of fasciola gigantica infection using monoclonal antibody-based sandwich ELISA and Immunochromatographic Assay for detection of circulating cathepsin L1 Protease
Anuracpreeda P.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
Immunodiagnostic monoclonal antibody-based sandwich ELISA of fasciolosis by detection of Fasciola gigantica circulating fatty acid binding protein Immunodiagnosis of paramphistomosis using monoclonal antibody-based sandwich ELISA for detection of Paramphistomum gracile circulating 16 kDa antigen Monoclonal antibody against recombinant Fasciola gigantica cathepsin L1H could detect juvenile and adult cathepsin Ls of Fasciola gigantica Development of botulinum toxin detection assay by antibody capture ELISA Sensitivity improvement of immunochromatographic strip test for infectious myonecrosis virus detection Bead array for Listeria monocytogenes detection using specific monoclonal antibodies Pesticide analysis using nanoceria-coated paper-based devices as a detection platform Development of molecular approach based on PCR assay for detection of histamine producing bacteria A silicon nitride ISFET based immunosensor for Ag85B detection of tuberculosis Molecular detection of Brucella abortus using bscp31 and IS711 gene based pcr assay in cattle and buffalo (Buffalo Bulletin (Thailand),37(1))
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