คลังข้อมูลการวิจัยการเกษตรไทย

ผู้แต่ง Benjawan Phetsuksiri

เผยแพร่โดย

1 National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Thailand 2 The Office of Disease Prevention and Control 2 Phitsanulok , Department of Disease Control 3 The Office of Disease Prevention and Control 1 Chiang Mai, Department of Disease Control 4 Regional Medical Sciences Center 1 Chiang Mai, Department of Medical Sciences 5 Regional Medical Sciences Center 7 Khon Kaen, Department of Medical Sciences

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งานวิจัยที่แนะนำ การตรวจหาเชื้อก่อโรคอุจจาระร่วงเฉียบพลัน 19 ชนิด ด้วยวิธี Quantitative real-time PCR Simultaneous detection and quantification of 19 diarrhea-related pathogens with a quantitative real-time PCR panel การตรวจหาเชื้อไวรัสและแบคทีเรียก่อโรคอุจจาระร่วงเฉียบพลัน 19 ชนิด ในการทดสอบเดียว ด้วยเทคนิค Multiplex real-time PCR Detection of 19 types of virus and bacteria causing acute diarrhea in single test b Simultaneous detection and quantification of 19 diarrhea-related pathogens with a quantitative real-time PCR panel assay Rapid molecular detection of multidrug- resistant tuberculosis by PCR-nucleic acid lateral flow immunoassay Development of Zika virus detection method by real time RT-PCR for using in Thailand Feasibility of high resolution melting curve analysis for rapid serotyping of Salmonella from hospitalised patients Development of the cost and time saving Zika virus detection method by real time RT-PCR in response for 2016 outbreak in Thailand Comparison of the Performances of Five Primer Sets for the Detection and Quantification of Plasmodium in Anopheline Vectors by Real-Time PCR Development and evaluation of multiplex real-time RT-PCR for Dengue, Chikungunya and Zika virus detection in Thailand Development of Mayaro virus detection by real-time RT-PCR and retrospective surveillance of incident in Thailand during 2016-2019

คัดลอก URL

ชื่อเรื่อง (EN):	Rapid detection of tuberculosis resistant to isoniazid and rifampicin by real-time PCR and melting curve analysis
ผู้แต่ง / หัวหน้าโครงการ (EN):	Benjawan Phetsuksiri
บทคัดย่อ (EN):	Tuberculosis (TB) is a chronic disease and drug resistant tuberculosis is considered a serious public health treat. The treatment of multi-drug resistant tuberculosis (MDR-TB) in which M. tuberculosis poses resistance to at least isoniazid and rifampicin, major ant- tuberculosis drugs is difficult and time consuming. Drug-susceptibility testing (DST) for Mycobacterium tuberculosis (MTB) is important for the effective treatment of tuberculosis (TB). This study assessed the real-time PCR method for rapid detection of drug-resistant tuberculosis (DR-TB). The assay was able to detect MTB resistant to isoniazid (INH) and rifampicin (RMP) simultaneously based on the analysis of DNA mutations. A total of 148 MTB clinical isolates having the results of DST, a standard method based on culture, were selected and subjected to real-time PCR analysis. A comparison of real-time PCR results and DST data showed a concordance, 54 of 54 (100%) in susceptible strains, 43 of 50 (86%) for INH resistance, 5 of 6 for RMP resistance and 34 of 38 isolates (89%) for multidrug-resistant TB (MDR-TB). Comparison of detection results by real-time PCR and DST, 12 samples showed discrepancy results. Further analysis revealed concordance with sequencing analysis in 11 of 12 samples. In conclusion, the method is rapid and suits for routine use in rapid detection of drug resistant TB. The detection result provides useful information in the initiation of TB treatment.
บทคัดย่อ:	ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN):	en
เอกสารแนบ (EN):	http://nih.dmsc.moph.go.th/research/showimgdetil.php?id=504
เผยแพร่โดย (EN):	1 National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Thailand 2 The Office of Disease Prevention and Control 2 Phitsanulok , Department of Disease Control 3 The Office of Disease Prevention and Control 1 Chiang Mai, Department of Disease Control 4 Regional Medical Sciences Center 1 Chiang Mai, Department of Medical Sciences 5 Regional Medical Sciences Center 7 Khon Kaen, Department of Medical Sciences
คำสำคัญ (EN):	rifampicin
เจ้าของลิขสิทธิ์ (EN):	National Institute of Health, Department of Medical Science