คลังข้อมูลการวิจัยการเกษตรไทย

ผู้แต่ง Jakkrawarn Chompoosri

เผยแพร่โดย

1 National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand 2 Department of Virology and Parasitology, Fujita Health University School of Medicine, Toyoake, Aichi, Japan 3 Department of Microbiology, Fujita Health University School of Medicine, Toyoake, Aichi, Japan 4 Department of Disease Control, Ministry of Public Health, Nonthaburi, Thailand 5 Department of Pediatrics, Fujita Health University School of Medicine, Toyoake, Aichi, Japan

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หน่วยงาน ไม่ระบุหน่วยงาน

งานวิจัยที่แนะนำ Environmental Factors Associated with Buildup of High Density Aedes Albopictus Vector of Chikunguniya Virus in Rubber Plantations Induction of defensin response to dengue infection in Aedes aegypti Norovirus transmission mediated by asymptomatic family members in households Characterization of chikungunya virus-like particles Comparative effectiveness of monomolecular surface film on Aedes aegypti (L.) and Anopheles minimus (Theobald) (Diptera: Culicidae) Efficacy of lemongrass aerosol sprays against insecticide-resistant Aedes aegypti mosquitoes from dengue risk areas in Nakhon Pathom and Chanthaburi Provinces Physical characteristics and reproductive performance in Aedes (Diptera: Culicidae) Insecticide susceptibility characterization and efficacy of insecticides for thermal space spray application against insecticide-resistant Aedes aegypti (L.) from Chanthaburi Province Synergistic effect of combination deltamethrin and piperonyl butoxide on behavioral avoidance and mortality responses of Aedes aegypti L. Larvicidal effect of compounds isolated from Maerua siamensis (Capparidaceae) against Aedes aegypti (Diptera: Culicidae) larvae

คัดลอก URL

ชื่อเรื่อง (EN):	Vertical transmission of Indian Ocean Lineage of chikungunya virus in Aedes aegypti and Aedes albopictus mosquitoes
ผู้แต่ง / หัวหน้าโครงการ (EN):	Jakkrawarn Chompoosri
บทคัดย่อ (EN):	Background: The re-emergence of chikungunya (CHIK) fever in Thailand has been caused by a novel lineage of chikungunya virus (CHIKV) termed the Indian Ocean Lineage (IOL). The Aedes albopictus mosquito is thought to be a primary vector of CHIK fever in Thailand, whereas Ae. aegypti acts as a secondary vector of the virus. The vertical transmission is believed to be a primary means to maintain CHIKV in nature and may be associated with an increased risk of outbreak. Therefore, the goal of this study was to analyze the potential of these two Thai mosquito species to transmit the virus vertically and to determine the number of successive mosquito generations for the virus transmission. Methods: Two-hundred-and-fifty female Ae. aegypti and Ae. albopictus mosquitoes were artificially fed a mixture of human blood and CHIKV IOL. Mosquito larvae and adults were sampled and screened for CHIKV by one-step qRT-PCR. LLC-MK2 cell line was used to isolate CHIKV in the mosquitoes each generation. The virus isolate was identified by immunocytochemical staining and was confirmed by sequencing. Both mosquito species fed on human blood without CHIKV and uninfected LLC-MK2 cells were used as controls. Results: Aedes aegypti and Ae. albopictus mosquitoes were able to transmit CHIKV vertically to F5 and F6 progenies, respectively. The virus isolated from the two mosquito species caused cytopathic effect in LLC-MK2 cells by 2 days post-infection and immunocytochemical staining showed the reaction between CHIKV IOL antigen and specific monoclonal antibody in the infected cells. DNA sequence confirmed the virus transmitted vertically as CHIKV IOL with E1-A226V mutation. No CHIKV infection was observed in both mosquito species and LLC-MK2 cells from control groups. Conclusions: The study demonstrated that Ae. aegypti and Ae. albopictus mosquitoes from Thailand are capable of transmitting CHIKV IOL vertically in the laboratory. Our results showed that Ae. albopictus is more susceptible and has a greater ability to transmit the virus vertically than Ae. aegypti. This knowledge would be useful for risk assessments of the maintenance of CHIKV in nature, which is crucial for disease surveillance, vector control and the prevention of potential CHIKV epidemics.
บทคัดย่อ:	ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN):	en
เอกสารแนบ (EN):	http://nih.dmsc.moph.go.th/research/showimgdetil.php?id=489
เผยแพร่โดย (EN):	1 National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand 2 Department of Virology and Parasitology, Fujita Health University School of Medicine, Toyoake, Aichi, Japan 3 Department of Microbiology, Fujita Health University School of Medicine, Toyoake, Aichi, Japan 4 Department of Disease Control, Ministry of Public Health, Nonthaburi, Thailand 5 Department of Pediatrics, Fujita Health University School of Medicine, Toyoake, Aichi, Japan
คำสำคัญ (EN):	Vertical transmission
เจ้าของลิขสิทธิ์ (EN):	National Institute of Health, Department of Medical Science