สืบค้นงานวิจัย
Dissection of gene loci underlying pasting temperature in cassava
Srisawad N. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Dissection of gene loci underlying pasting temperature in cassava
ผู้แต่ง / หัวหน้าโครงการ (EN): Srisawad N.
บทคัดย่อ (EN): In this study, a fine genetic map within the quantitative trait loci (QTL) underlying pasting temperature (PT) of cassava (Manihot esculenta Crantz) was constructed using newly developed simple sequence repeat (SSR) markers. The SSRs were designed on the basis of two scaffolds (S11341 and S4043) of the cassava genome, which covered previously identified QTL regions of the PT trait. A total of 55 and 61 SSR markers derived from S11341 and S4043, respectively, representing 0.29% of the cassava genome, were generated; of which 23 and 19 showed informative polymorphic patterns. Consequently, all identified informative polymorphic markers were used to genotype 200 F1 progeny plants. The genotypic data were then analyzed, and the results showed that 480 markers were distributed across 23 linkage groups (LGs) with total length of 1,334 centimorgans (cM). An analysis of QTL underlying the PT trait revealed that marker EME81 on LG 1 had significant associations (P < 0.0001) in all environments evaluated. Four candidate genes were identified and selected for gene expression analysis in the parents, and among F1 lines with high and low PT values. Significant differences were observed in relative expression of carbohydrate phosphorylase (CP) and starch synthase II (SSII) between high and low PT in 6-month-old cassava. We found CP and SSII genes to potentially control the PT trait. In addition, the marker EME81 was found to be a promising marker for specific PT trait selection in cassava populations, which should facilitate marker-assisted selection for desired PT traits. © 2018 Informa UK Limited, trading as Taylor & Francis Group.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-85045028520&doi=10.1080%2f15427528.2018.1458364&partnerID=40&md5=b250390048cc13f85ee4ee12a8a1a5d0
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): simple sequence repeat (SSR) markers
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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Srisawad N.. "Dissection of gene loci underlying pasting temperature in cassava". ไม่ระบุหน่วยงาน. ม.ป.ป.

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Dissection of gene loci underlying pasting temperature in cassava
Srisawad N.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
Quantitative trait loci underlying root yield and starch content in an F1 derived cassava population (Manihot esculenta Crantz) Mapping of quantitative trait loci underlying resistance to cassava anthracnose disease Quantitative trait loci and candidate genes associated with starch pasting viscosity characteristics in cassava (Manihot esculenta Crantz) Phenotypic variation of cassava root traits and their responses to drought Identification of Differentially Expressed Proteins in Cassava Infected with Colletotrichum gloeosporioides f. sp. manihotis Effect of temperature on fimbrial gene expression and adherence of enteroaggregative Escherichia coli Isolation and Characterization of Microsatellite Loci and Genetic Diversity in Cassava (Manihot esculenta Crantz) Effect of fertilizer rates on cassava grown on Yasothon soil amended with cassava stem base biochar and wastes from cassava starch manufacturing plant Genome-wide analysis of aquaporin gene family and their responses to water-deficit stress conditions in cassava Gene co-expression analysis inferring the crosstalk of ethylene and gibberellin in modulating the transcriptional acclimation of cassava root growth in different seasons
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