สืบค้นงานวิจัย
Analysis of Ascorbic Acid and Isoascorbic Acid in Orange and Guava Fruit Juices Distributed in Thailand by LC-IT-MS/MS
Boonpangrak S. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Analysis of Ascorbic Acid and Isoascorbic Acid in Orange and Guava Fruit Juices Distributed in Thailand by LC-IT-MS/MS
ผู้แต่ง / หัวหน้าโครงการ (EN): Boonpangrak S.
บทคัดย่อ (EN): This work describes the determination of ascorbic acid (AA) and isoascorbic acid (IAA) in fruit juices by liquid chromatography coupled with ion trap tandem mass spectrometry (LC-IT-MS/MS), with a reversed phase column (C18) and simple isocratic conditions of 0.1 % formic acid. The negative ion mode of electrospray ionization (ESI) and the MS/MS transition of m/z 175 were used for the quantitation of AA- and IAA-generated product ions with m/z 115. The method was validated in terms of linearity, limit of detection (LOD), limit of quantitation (LOQ), precision, and accuracy. Good linearity was achieved with 0.6 and 1.8 μg/mL for the LOD and LOQ, respectively. The intraday and intermediate precisions were approximately 4 and 7 %, respectively. Recovery percentages ranged from 88 to 108 %. All validation parameters were found to be within the acceptable range for both AA and IAA. Hence, the proposed method is suitable for simultaneously determining AA and IAA. Fourteen fruit juice samples were analyzed including fruit juices from supermarkets, local markets, and laboratory preparations. Ten fruit juice samples (100 %) from different brands in the supermarkets were investigated for AA and IAA content. AA could be detected in all the samples, ranging from 5.2 ± 2 to 44.4 ± 1.3 μg/mL, and the values of vitamin C in 4 guava samples were less than the values specified by the manufacturer. IAA was observed in 4 of 10 samples ranging from 3.4 ± 0.9 to 78.3 ± 3.9 μg/mL, and the highest value of IAA was approximately two fold higher than the highest value of AA. For freshly prepared fruit juices, AA was detected in both local market- and laboratory-prepared juices. The value ranged from 13.2 ± 0.9 to 105.1 ± 1.3 μg/mL. In addition, the AA stability after opening the package was evaluated. The results showed that after 4 days of storage in the refrigerator, approximately 30 and 15 % losses of AA were observed for the orange and guava juices, respectively. Fresh fruit juices were thus demonstrated to be good sources of AA, with the highest value observed for guava juice prepared in the laboratory. © 2015, Springer Science+Business Media New York.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-84945157097&doi=10.1007%2fs12161-015-0337-x&partnerID=40&md5=479dbd9c08b014ec6689a3d6beb8be35
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Ascorbic acid
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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การอ้างอิง

Boonpangrak S.. "Analysis of Ascorbic Acid and Isoascorbic Acid in Orange and Guava Fruit Juices Distributed in Thailand by LC-IT-MS/MS". ไม่ระบุหน่วยงาน. ม.ป.ป.

Boonpangrak S.. "Analysis of Ascorbic Acid and Isoascorbic Acid in Orange and Guava Fruit Juices Distributed in Thailand by LC-IT-MS/MS". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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Analysis of Ascorbic Acid and Isoascorbic Acid in Orange and Guava Fruit Juices Distributed in Thailand by LC-IT-MS/MS
Boonpangrak S.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
Development and validation of LC-MS/MS method for quantitative determination of adenosine, guanosine, xanthine and uric acid in widely consumed vegetables in Thailand การวิเคราะห์สารอัลคาลอยด์มัสคาลีนในเห็ดพิษด้วยวิธี Liquid Chromatography-tandem Mass Spectrometry (LC-MS/MS) An Analysis of alkaloid muscarine-containing mushrooms using liquid chromatography-tandem m Foodborne illness caused by muscarine-containing mushrooms and identification of mushroom remnants using phylogenetics and LC-MS/MS Appropriate option of market solid waste management based on the stakeholder perspectives: A case study in a central market of agriculture products, Thailand Qualitative and quantitative comparison of the proteome of erythroid cells differentiated from human iPSCs and adult erythroid cells by multiplex TMT labelling and nanoLC-MS/MS Successive detection of benzoic acid and total parabens in foodstuffs using mercaptosuccinic acid capped cadmium telluride quantum dots Components Analysis of Different Solvents Extracts from Rhizosphere soil of Rubber Tree by GC－MS การปรับปรุงพันธุ์ข้าวโพดให้มีลักษณะ low-phytic acid Two-step acid and alkaline ethanolysis/alkaline peroxide fractionation of sugarcane bagasse and rice straw for production of polylactic acid precursor Isolation of hippuric acid from buffalo urine and its antioxidant activity (Buffalo Bulletin (Thailand),36(3))
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