สืบค้นงานวิจัย
Over-Expression of Chitinase Gene and In Vitro Antifungal Activity of Recombinant Chitinase Protein Against Corynespora Cassiicola Infection Rebber (Hevea brasiliensis)
Shaji Hpilip - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Over-Expression of Chitinase Gene and In Vitro Antifungal Activity of Recombinant Chitinase Protein Against Corynespora Cassiicola Infection Rebber (Hevea brasiliensis)
ผู้แต่ง / หัวหน้าโครงการ (EN): Shaji Hpilip
บทคัดย่อ (EN): Corynespora leaf disease caused by Corynespora cassiicola has emerged as a major disease of rubber in South East Asia. During plant-plant-pathogen interacions, various novel proteins called pathogenesis related (PR) proteins which play a major role in plant disease resistance mechanism are induced. The chitinase (PR3) is one of the most widely studied groups of PR proteins in plants during pathogenesis. Corynespora cassiicola induced chitinase from Hevea brasiliensis was characterized in the present study. The cDNA was developed from the RNA of C.cassiicola infected leaves of the clone GT1. A 978 bp chitinase gene was obtained from H.brasiliensis and over-expressed in the pET 32a+ expression system. In vitro studies of purified recombinant Hevea chitinase showed antifungal activity against C. cassiicola. The chitinase gene expression in Hevea brasiliensis during Corynespora cassiicola infection was quantified through qPCR and increased expression of chitinase transcripts was observed. In clone GT1, chitinase gene was induced up to 24 hours after Corynespora cassiicola infection and then came down, where as in RRII 105, chitinase level was lesser induced than control. The polyclonal antibody was raised with the recombinant chitinase and the induced clone GTI showed a prominent vand in western blot, While an minor band was observed in RRII 105 in induced condition.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เผยแพร่โดย (EN): การยางแห่งประเทศไทย
คำสำคัญ (EN): Corynespora cassiicola
เจ้าของลิขสิทธิ์ (EN): การยางแห่งประเทศไทย
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Shaji Hpilip. "Over-Expression of Chitinase Gene and In Vitro Antifungal Activity of Recombinant Chitinase Protein Against Corynespora Cassiicola Infection Rebber (Hevea brasiliensis)". ไม่ระบุหน่วยงาน. ม.ป.ป.

Shaji Hpilip. "Over-Expression of Chitinase Gene and In Vitro Antifungal Activity of Recombinant Chitinase Protein Against Corynespora Cassiicola Infection Rebber (Hevea brasiliensis)". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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Over-Expression of Chitinase Gene and In Vitro Antifungal Activity of Recombinant Chitinase Protein Against Corynespora Cassiicola Infection Rebber (Hevea brasiliensis)
Shaji Hpilip
การยางแห่งประเทศไทย
ไม่ระบุวันที่เผยแพร่
CDNA –AFLP ANALYSIS REVEALS DIFFERENTIAL GENE EXPRESSION IN RUBBER TREE (HEVEA BRASILIENSIS) INOCULATED WITH CORYNESPORA CASSIICOLA PATHOGEN Chlorophy11 A/B Binding Protein Gene Expression in Juvenile and Mature Leaf Explants and Its Relationship with In Vitro Culture Response in Hevea brasiliensis Structural and Functional Characterization of a NBS-LRR Disease Resistance Gene Associated With Tolerance against Corynespora Cassiicola Causing Leaf Fall Disease in Rubber (Hevea brasiliensis) Generation of Tranceriptome Recources in Rubber (Hevea brasiliensis) in Response to Corynespora Cassiicola Causing Corynespora Leaf Disease for Gene Discovery and Marker Identification Using Ngs Platf Rapid Molecular Identification and Detection of Corynespora cassiicola of Hevea brasiliensis Functional Characterization of a Novel Hevea â-1, 3-Glucanase Gene Promoter and Its Regulatory Role in Hevea and Tobacco Tissues Surveillance on Corynespora Leaf Fall Disease: Incidence and Severity on Natural Rubber (Hevea brasiliensis) in Certain Hot Spots Areas in Kerala Expression of NAC Transcription Factor under Drought Stress in Hevea brasiliensis IMPROVEMENT AND APPLICATION OF THE TECHNIQUE OF MINI-SEEDLING BUDDING OF HEVEA BRASILIENSIS CLONAL VARIABILITY IN THE INCLINATION OF LATICIFERS AND PHLOIC RAYS IN HEVEA BRASILIENSIS
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