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Characterization of an abalone gonadotropin-releasing hormone and its effect on ovarian cell proliferation
Nuurai P. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Characterization of an abalone gonadotropin-releasing hormone and its effect on ovarian cell proliferation
ผู้แต่ง / หัวหน้าโครงการ (EN): Nuurai P.
บทคัดย่อ (EN): In various animal groups, both vertebrates and invertebrates, it has been reported that gonadotropin-releasing hormone (GnRH) is an important regulator of reproductive function. Knowledge of the molecular identity of a GnRH and its function in the abalone is unknown. In this study, we have characterized the abalone GnRH genes and their putative precursor sequences from the neural ganglia of sexually mature female Haliotis asinina and Haliotis laevigata. The abalone GnRH genes demonstrate high similarity with other gastropods within the proposed bioactive GnRH peptide regions (H. asinina: QNYHFSNGWYPG; H. laevigata: QNYHFSNGWHA) but less in the GnRH-associated peptide region. An antibody generated against HasGnRH showed specific expression of the peptide within the cerebral and pleuropedal ganglia. HasGnRH was localized in type-1 neurosecretory cells that are widely distributed within the ventral and dorsal parts of the cerebral ganglion and also observed in nerve fiber bundles extending into the dorsal part of the ganglion. In the pleuropedal ganglion, HasGnRH immunoreactive cells were distributed in both ventral and dorsal horns. Nerve fibers containing HasGnRH immunoreactivity were also detected primarily within the ventral region of ventral horns of the ganglion. Synthetic HasGnRH and buserelin stimulated oogonia and oocyte proliferation in one-year-old female H. asinina as determined by in vivo and in vitro BrdU ovarian cell proliferation. These findings provide important knowledge into the molecular identity of the abalone GnRH and its function in stimulating oocyte proliferation that helps us to further understand the role of this peptide in molluscs which may be applied to aquaculture of this species. © 2015 Elsevier B.V..
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-84939430545&doi=10.1016%2fj.aquaculture.2015.07.008&partnerID=40&md5=f4755893fe4b4fcce83c2349f9073f28
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Vertebrata
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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Characterization of an abalone gonadotropin-releasing hormone and its effect on ovarian cell proliferation
Nuurai P.
มหาวิทยาลัยมหิดล
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