สืบค้นงานวิจัย
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for the identification of Burkholderia pseudomallei from Asia and Australia and differentiation between Burkholderia species
Suttisunhakul V. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for the identification of Burkholderia pseudomallei from Asia and Australia and differentiation between Burkholderia species
ผู้แต่ง / หัวหน้าโครงการ (EN): Suttisunhakul V.
บทคัดย่อ (EN): Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is increasingly used for rapid bacterial identification. Studies of Burkholderia pseudomallei identification have involved small isolate numbers drawn from a restricted geographic region. There is a need to expand the reference database and evaluate B. pseudomallei from a wider geographic distribution that more fully captures the extensive genetic diversity of this species. Here, we describe the evaluation of over 650 isolates. Main spectral profiles (MSP) for 26 isolates of B. pseudomallei (N = 5) and other Burkholderia species (N = 21) were added to the Biotyper database. MALDI-TOF MS was then performed on 581 B. pseudomallei, 19 B. mallei, 6 B. thailandensis and 23 isolates representing a range of other bacterial species. B. pseudomallei originated from northeast and east Thailand (N = 524), Laos (N = 12), Cambodia (N = 14), Hong Kong (N = 4) and Australia (N = 27). All 581 B. pseudomallei were correctly identified, with 100% sensitivity and specificity. Accurate identification required a minimum inoculum of 5 × 107 CFU/ml, and identification could be performed on spiked blood cultures after 24 hours of incubation. Comparison between a dendrogram constructed from MALDI-TOF MS main spectrum profiles and a phylogenetic tree based on recA gene sequencing demonstrated that MALDI-TOF MS distinguished between B. pseudomallei and B. mallei, while the recA tree did not. MALDI-TOF MS is an accurate method for the identification of B. pseudomallei, and discriminates between this and other related Burkholderia species. © 2017 Suttisunhakul et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-85017112646&doi=10.1371%2fjournal.pone.0175294&partnerID=40&md5=cb491bcc2b5dd8811a2fea616bb4a101
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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Suttisunhakul V.. "Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for the identification of Burkholderia pseudomallei from Asia and Australia and differentiation between Burkholderia species". ไม่ระบุหน่วยงาน. ม.ป.ป.

Suttisunhakul V.. "Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for the identification of Burkholderia pseudomallei from Asia and Australia and differentiation between Burkholderia species". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for the identification of Burkholderia pseudomallei from Asia and Australia and differentiation between Burkholderia species
Suttisunhakul V.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
Source-identifying biomarker ions between environmental and clinical Burkholderia pseudomallei using whole-cell Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF A new endemic Thai species of Argyreia (Convolvulaceae) Immune response to recombinant Burkholderia pseudomallei FliC Utilization of whole-cell MALDI-TOF mass spectrometry to differentiate Burkholderia pseudomallei wild-type and constructed mutants Soil nutrient depletion is associated with the presence of Burkholderia pseudomallei Distribution and Ecological Drivers of Spotted Fever Group Rickettsia in Asia The appearance of allelopathic substances affecting the presence of Burkholderia pseudomallei Multitarget quantitative PCR improves detection and predicts cultivability of the pathogen Burkholderia pseudomallei Analysis of the prevalence, secretion and function of a cell cycle-inhibiting factor in the melioidosis pathogen Burkholderia pseudomallei การเฝ้าระวังเชื้อ Burkholderia pseudomallei ในสัตว์จากโรงฆ่าสัตว์ในจังหวัดนครปฐมและราชบุรี
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