สืบค้นงานวิจัย
Functional characterization of metallothionein-like genes from Physcomitrella patens: expression profiling, yeast heterologous expression, and disruption of PpMT1.2a gene
Pakdee O. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Functional characterization of metallothionein-like genes from Physcomitrella patens: expression profiling, yeast heterologous expression, and disruption of PpMT1.2a gene
ผู้แต่ง / หัวหน้าโครงการ (EN): Pakdee O.
บทคัดย่อ (EN): Main conclusion: Physcomitrella patens contains four metallothionein-like genes. Three were shown to confer metal tolerance in yeast. Transcript profiling suggests their roles in senescence and reproductive development or cadmium and oxidative stress. Abstract: Metallothioneins (MTs) have been suggested to play various roles including metal detoxification, nutrient remobilization, ROS scavenging, stress tolerance, and plant development. However, little is known about the forms and functions of MTs in bryophytes. The moss Physcomitrella patens genome was found to contain four MT-like genes. Amino acid sequence composition showed that the P. patens MTs (PpMTs) were clustered with Type 1 plant MTs, and could be further classified into two sub-types, herein referred to as sub-type 1: PpMT1.1a and PpMT1.1b and sub-type 2: PpMT1.2a and PpMT1.2b. Transcript abundance of PpMT1.1b and PpMT1.2b was upregulated in the gametophore compared to protonema, and all, except PpMT1.2a, were highly induced in senescing gametophytes. PpMT1.1a and PpMT1.1b transcripts were upregulated in protonema treated with cadmium and hydrogen peroxide. Unlike many higher plant MTs, the PpMT transcript abundance was not strongly induced in response to copper and zinc. These results suggest that PpMTs may play a role in protecting P. patens from cadmium and oxidative stress and may be involved in tissues senescence and reproductive development. The PpMTs, except PpMT1.2b, were also able to confer metal tolerance and accumulation when heterologously expressed in the ∆cup1 yeast. A P. patens mutant lacking PpMT1.2a through targeted gene disruption was generated. However, it did not show any alteration in growth phenotypes under senescence-induced conditions or hypersensitivity to cadmium, copper, zinc, H 2 O 2 , and NaCl stresses. Further characterization of additional P. patens mutants lacking single or multiple PpMTs may provide insight into the physiological roles of bryophytic MTs. © 2019, Springer-Verlag GmbH Germany, part of Springer Nature.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-85065167334&doi=10.1007%2fs00425-019-03173-8&partnerID=40&md5=acc915aeb5a3c4ef382067dd321b893a
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Oxidative stress
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
หากไม่พบเอกสารฉบับเต็ม (Full Text) โปรดติดต่อหน่วยงานเจ้าของข้อมูล

การอ้างอิง

Pakdee O.. "Functional characterization of metallothionein-like genes from Physcomitrella patens: expression profiling, yeast heterologous expression, and disruption of PpMT1.2a gene". ไม่ระบุหน่วยงาน. ม.ป.ป.

Pakdee O.. "Functional characterization of metallothionein-like genes from Physcomitrella patens: expression profiling, yeast heterologous expression, and disruption of PpMT1.2a gene". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



TARR Wordcloud:
Functional characterization of metallothionein-like genes from Physcomitrella patens: expression profiling, yeast heterologous expression, and disruption of PpMT1.2a gene
Pakdee O.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
Gene expression and promoter characterization of heat-shock protein 90B gene (HSP90B) in the model unicellular green alga Chlamydomonas reinhardtii AQUAPORIN GENES EXPRESSION IN THE TRUNK PHLOEM AND IN THE LATICIFERS OF RUBBER TREE Expression and characterization of glutathione peroxidase of the liver fluke, Fasciola gigantica Expression divergence of chemosensory genes between Drosophila sechellia and its sibling species and its implications for host shift Effect of temperature on fimbrial gene expression and adherence of enteroaggregative Escherichia coli IDENTIFICATION OF DROUGHT TOLERANT GENES BY QUANTITATIVE EXPRESSION ANALYSIS IN HEVEA BRASILIENSIS Blood-induced differential gene expression in Anopheles dirus evaluated using RNA sequencing Cloning of Arabidopsis WUS Gene, Construcion and Identification of WUS-EGFP Fusion Gene Plant Expression Vector Cloning, expression, purification and characterization of a thermo- and surfactant-stable protease from Thermomonospora curvata Expression of expansin genes in the pulp and the dehiscence zone of ripening durian (Durio zibethinus) fruit
คัดลอก URL
กระทู้ของฉัน
ผลการสืบค้นทั้งหมด โพสต์     เรียงลำดับจาก
พัฒนาระบบโดยศูนย์ความรู้เฉพาะด้านวิศวกรรมความรู้และวิศวกรรมภาษาDeveloped by Center of Excellence for Unified Knowledge and Language Engineering
สนับสนุนโดยสำนักงานพัฒนาการวิจัยการเกษตร (องค์การมหาชน)Supported by the Agricultural Research Development Agency (Public Organization)
แบบสำรวจความพึงพอใจQuestionair