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Complete mitochondrial genome reveals genetic diversity of Angiostrongylus cantonensis (Nematoda: Angiostrongylidae)
Yong H.-S. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Complete mitochondrial genome reveals genetic diversity of Angiostrongylus cantonensis (Nematoda: Angiostrongylidae)
ผู้แต่ง / หัวหน้าโครงการ (EN): Yong H.-S.
บทคัดย่อ (EN): Angiostrongylus cantonensis is a zoonotic parasite that causes eosinophilic meningitis in humans. Earlier work on its mitochondrial genome was based on long polymerase chain reaction method. To date, only the mitogenome of the isolates from China has been studied. We report here the complete mitogenome of the Thailand isolate based on next generation sequencing and compare the genetic diversity with other isolates. The mitogenome of the Thailand isolate (13,519 bp) is longer than those of the China isolates (13,497-13,502 bp). Five protein-coding genes (atp6, cox1, cox2, cob, nad2) show variations in length among the isolates. The stop codon of the Thailand isolate differs from the China and Taiwan isolates in 4 genes (atp6, cob, nad2, nad6). Additionally, the Thailand isolate has 4 incomplete T stop codon compared to 3 in the China and Taiwan isolates. The control region is longer in the Thailand isolate (258 bp) than the China (230-236 bp) and Taiwan (237 bp) isolates. The intergenic sequence between nad4 and cox1 genes in the Thailand isolate lacks 2 bp (indels) at the 5'-end of the sequence as well as differs at 7 other sites compared to the China and Taiwan isolates. In the Thailand isolate, 18 tRNAs lack the entire TΨC-arm, compared to 17 in the China isolate and 16 in the Taiwan isolate. Phylogenetic analyses based on 36 mt-genes, 12 PCGs, 2 rRNA genes, 22 tRNA genes and control region all indicate closer genetic affinity between the China and Taiwan isolates compared to the Thailand isolate. Based on 36 mt-genes, the inter-isolate genetic distance varies from p= 3.2% between China and Taiwan isolates to p= 11.6% between Thailand and China isolates. The mitogenome will be useful for population, phylogenetics and phylogeography studies. © 2015 Elsevier B.V.
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ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-84942582035&doi=10.1016%2fj.actatropica.2015.09.001&partnerID=40&md5=d025d1637f25a34c932c594c65f50bbb
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): RNA, Ribosomal
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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Complete mitochondrial genome reveals genetic diversity of Angiostrongylus cantonensis (Nematoda: Angiostrongylidae)
Yong H.-S.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
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