สืบค้นงานวิจัย
Identification of new regions in HIV-1 gp120 Variable 2 and 3 Loops that Bind to α4β7 Integrin Receptor
Peachman K.K. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Identification of new regions in HIV-1 gp120 Variable 2 and 3 Loops that Bind to α4β7 Integrin Receptor
ผู้แต่ง / หัวหน้าโครงการ (EN): Peachman K.K.
บทคัดย่อ (EN): The gut mucosal homing integrin receptor α4β7 present on activated CD4+ T cells interacts with the HIV-1 gp120 second variable loop (V2). Case control analysis of the RV144 phase III vaccine trial demonstrated that plasma IgG binding antibodies specific to scaffolded proteins expressing the first and second variable regions (V1V2) of HIV envelope protein gp120 containing the α4β7 binding motif correlated inversely with risk of infection. Subsequently antibodies to the V3 region were also shown to correlate with protection. The integrin receptor α4β7 was shown to interact with the LDI/V motif on V2 loop but recent studies suggest that additional regions of V2 loop could interact with the α4β7. Thus, there may be several regions on the V2 and possibly V3 loops that may be involved in this binding. Using a cell line, that constitutively expressed α4β7 receptors but lacked CD4, we examined the contribution of V2 and V3 loops and the ability of V2 peptide-, V2 integrin-, V3-specific monoclonal antibodies (mAbs), and purified IgG from RV144 vaccinees to block the V2/V3-α4β7 interaction. Results We demonstrate that α4β7 on RPMI8866 cells bound specifically to its natural ligand mucosal addressin cell adhesion molecule-1 (MAdCAM-1) as well as to cyclic-V2 and cyclic-V3 peptides. This binding was inhibited by anti-α4β7-specific monoclonal antibody (mAb) ACT-1, mAbs specific to either V2 or V3 loops, and by purified primary virions or infectious molecular clones expressing envelopes from acute or chronic subtypes A, C, and CRF01-AE viruses. Plasma from HIV-1 infected Thai individuals as well as purified IgG from uninfected RV144 vaccinees inhibited (0-50%) the binding of V2 and V3 peptides to α4β7. Conclusion Our results indicate that in addition to the tripeptide LDI/V motif, other regions of the V2 and V3 loops of gp120 were involved in binding to α4β7 receptors and this interaction was blocked by anti-V2 peptide, anti-V2 integrin, and anti-V3 antibodies. The ability of purified IgG from some of the uninfected RV144 vaccinees to inhibit α4β7 raises the hypothesis that anti-V2 and anti-V3 antibodies may play a role in blocking the gp120-α4β7 interaction after vaccination and thus prevent HIV-1 acquisition.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-84961356295&doi=10.1371%2fjournal.pone.0143895&partnerID=40&md5=2e8ed23f4d7113caf7a943c8ab6f86d9
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): human cell
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
หากไม่พบเอกสารฉบับเต็ม (Full Text) โปรดติดต่อหน่วยงานเจ้าของข้อมูล

การอ้างอิง

Peachman K.K.. "Identification of new regions in HIV-1 gp120 Variable 2 and 3 Loops that Bind to α4β7 Integrin Receptor". ไม่ระบุหน่วยงาน. ม.ป.ป.

Peachman K.K.. "Identification of new regions in HIV-1 gp120 Variable 2 and 3 Loops that Bind to α4β7 Integrin Receptor". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



TARR Wordcloud:
Identification of new regions in HIV-1 gp120 Variable 2 and 3 Loops that Bind to α4β7 Integrin Receptor
Peachman K.K.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
Cryptic determinant of a4b7 binding in the v2 loop of hiv-1 gp120 Characterization of HIV-1 gp120 antibody specificities induced in anogenital secretions of RV144 vaccine recipients after late boost immunizations Glycans flanking the hypervariable connecting peptide between the a and B strands of the V1/V2 domain of HIV-1 gp120 confer resistance to antibodies that neutralize CRF01-AE viruses A new neolignan, and the cytotoxic and anti-HIV-1 activities of constituents from the roots of Dasymaschalon sootepense Prevalence of HIV infection, access to HIV care, and response to antiretroviral therapy among partners of HIV-infected individuals in Thailand HIV-1- specific IgA monoclonal antibodies from an HIV-1 vaccinee mediate galactosylceramide blocking and phagocytosis PATHOGEN IDENTIFICATION AND BIOLOGICAL CHARACTERISTICS OF A NEW STEM CANKER DISEASE ON RUBBER TREE National Survey of Pretreatment HIV-Drug Resistance in Thai HIV-1-Infected Adults Identification of immunodominant cd4-restricted epitopes co-located with antibody binding sites in individuals vaccinated with ALVAC-HIV and AIDSVAX B/E IDENTIFICATION OF FACTORS OF NATURAL RUBBER VARIABILITY TO DEVELOP NEW GRADES ADAPTED TO THE ANTIVIBRATION APPLICATION
คัดลอก URL
กระทู้ของฉัน
ผลการสืบค้นทั้งหมด โพสต์     เรียงลำดับจาก
พัฒนาระบบโดยศูนย์ความรู้เฉพาะด้านวิศวกรรมความรู้และวิศวกรรมภาษาDeveloped by Center of Excellence for Unified Knowledge and Language Engineering
สนับสนุนโดยสำนักงานพัฒนาการวิจัยการเกษตร (องค์การมหาชน)Supported by the Agricultural Research Development Agency (Public Organization)
แบบสำรวจความพึงพอใจQuestionair