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Novel identification of Dermacentor variabilis Arp2/3 complex and its role in rickettsial infection of the arthropod vector
Petchampai N. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Novel identification of Dermacentor variabilis Arp2/3 complex and its role in rickettsial infection of the arthropod vector
ผู้แต่ง / หัวหน้าโครงการ (EN): Petchampai N.
บทคัดย่อ (EN): Tick-borne spotted fever group (SFG) Rickettsia species must be able to infect both vertebrate and arthropod host cells. The host actin-related protein 2/3 (Arp2/3) complex is important in the invasion process and actin-based motility for several intracellular bacteria, including SFG Rickettsia in Drosophila and mammalian cells. To investigate the role of the tick Arp2/3 complex in tick-Rickettsia interactions, open reading frames of all subunits of the protein including Arp2, Arp3, ARPC1, ARPC2, ARPC3, ARPC4, and ARPC5 were identified from Dermacentor variabilis. Amino acid sequence analysis showed variation (ranging from 25-88%) in percent identity compared to the corresponding subunits of the complex from Drosophila melanogaster, Mus musculus, Homo sapiens, and Saccharomyces cerevisiae. Potential ATP binding sites were identified in D. variabilis (Dv) Arp2 and Arp3 subunits as well as five putative WD (Trp-Asp) motifs which were observed in DvARPC1. Transcriptional profiles of all subunits of the DvArp2/3 complex revealed greater mRNA expression in both Rickettsia-infected and -uninfected ovary compared to midgut and salivary glands. In response to R. montanensis infection of the tick ovary, the mRNA level of only DvARPC4 was significantly upregulated compared to uninfected tissues. Arp2/3 complex inhibition bioassays resulted in a decrease in the ability of R. montanensis to invade tick tissues with a significant difference in the tick ovary, indicating a role for the Arp2/3 complex in rickettsial invasion of tick cells. Characterization of tick-derived molecules associated with rickettsial infection is imperative in order to better comprehend the ecology of tickborne rickettsial diseases. © 2014 Petchampai et al.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-84899633225&doi=10.1371%2fjournal.pone.0093768&partnerID=40&md5=c2d92a7b0e041ece76264aa580162b01
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Sequence Analysis, DNA
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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Novel identification of Dermacentor variabilis Arp2/3 complex and its role in rickettsial infection of the arthropod vector
Petchampai N.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
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