สืบค้นงานวิจัย
Double-Loop-Mediated Isothermal Amplification (D-LAMP) using colourimetric gold nanoparticle probe for rapid detection of infectious Penaeus stylirostris densovirus (PstDNV) with reduced false-positive results from endogenous viral elements
Arunrut N. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Double-Loop-Mediated Isothermal Amplification (D-LAMP) using colourimetric gold nanoparticle probe for rapid detection of infectious Penaeus stylirostris densovirus (PstDNV) with reduced false-positive results from endogenous viral elements
ผู้แต่ง / หัวหน้าโครงการ (EN): Arunrut N.
บทคัดย่อ (EN): Endogenous viral elements (EVEs) of Penaeus stylirostris densovirus (PstDNV) in shrimp genome have been reported to occur randomly, and may cause false positive in diagnosis, because most of conventional detection methods only target a small nucleotide sequence of PstDNV. Since PstDNV is listed by the World Organization for Animal Health (OIE) as a reportable virus in crustacean, several countries require shrimp stocks imported for aquaculture to be screened for this virus. The rapidly and highly sensitive detection methods with reduced false positive from EVEs are necessary to guarantee and maintain absence of infectious PstDNV in traded stocks. To overcome problem posed by the viral insertions to shrimp genome, two set of primers were designed for LAMP combined with colourimetric gold nanoparticles (AuNPs), so-called D-LAMP, to detect two regions of PstDNV genome. The first target region targeted OIE-recommended nucleotide sequence of 1740–1968 (GenBank Accession no 273215) from our previous report. The second region targeted at the 3′end of the PstDNV genome that has been reported least likely to give rise to EVE in the shrimp genome. Detection sensitivity is approximately 100 copies. Results from LAMP combined with colourimetric AuNPs and conventional PCR methods are in agreement for detecting the infectious PstDNV. D-LAMP is also applicable to detect infectious type of PstDNV in P. stylirostris tissue sections (i.e. in situ DIG-labelled LAMP) and reaffirms its high specificity for detecting PstDNV infection. © 2019 Elsevier B.V.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-85066425146&doi=10.1016%2fj.aquaculture.2019.05.049&partnerID=40&md5=d458437ad7de547fe89867bcb67d64bc
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Litopenaeus stylirostris
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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Arunrut N.. "Double-Loop-Mediated Isothermal Amplification (D-LAMP) using colourimetric gold nanoparticle probe for rapid detection of infectious Penaeus stylirostris densovirus (PstDNV) with reduced false-positive results from endogenous viral elements". ไม่ระบุหน่วยงาน. ม.ป.ป.

Arunrut N.. "Double-Loop-Mediated Isothermal Amplification (D-LAMP) using colourimetric gold nanoparticle probe for rapid detection of infectious Penaeus stylirostris densovirus (PstDNV) with reduced false-positive results from endogenous viral elements". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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Double-Loop-Mediated Isothermal Amplification (D-LAMP) using colourimetric gold nanoparticle probe for rapid detection of infectious Penaeus stylirostris densovirus (PstDNV) with reduced false-positive results from endogenous viral elements
Arunrut N.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
Sensitive visual detection of AHPND bacteria using loop-mediated isothermal amplification combined with DNAFunctionalized gold nanoparticles as probes การพัฒนาวิธีการตรวจโรคเมลิออยโดสิสด้วย loop-mediated isothermal amplification (LAMP) Development of loop-mediated isothermal amplification (LAMP) for diagnosing melioidosis Development of the Rapid Test Kit for the Identification of Campylobacter spp. Based on Loop-mediated Isothermal Amplification (LAMP) in Combination with a Lateral Flow Dipstick (LFD) and Gold Nano-DN การพัฒนาวิธีการตรวจโรคเมลิออยโดสิสและเลปโตสไปโรสิสด้วย loop-mediated isothermal amplification (LAMP) Development of loop-mediated isothermal amplification (LAMP) for diagnosing melioidosis and leptosp Development of reverse transcription-loop-mediated isothermal amplification (RT-LAMP) for the detection of the causative agent of covid-19 Development of a rapid, simple method for detecting Naegleria fowleri visually in water samples by Loop-Mediated Isothermal Amplification (LAMP) The performance of in-house loop-mediated isothermal amplification for rapid detection of Mycobacterium tuberculosis in sputum sample comparing to Xpert MTB/RIF, microscopy and culture Pragmatic accuracy of an in-house loop-mediated isothermal amplification (LAMP) for diagnosis of pulmonary tuberculosis in a Thai community hospital Shewanella putrefaciens in cultured tilapia detected by a new calcein-loop-mediated isothermal amplification (Ca-LAMP) method การวินิจฉัยโรคติดเชื้อ 9 ชนิดในกุ้งทะเลอย่างรวดเร็วด้วยเทคนิค loop-mediated isothermal amplification (LAMP)
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