คลังข้อมูลการวิจัยการเกษตรไทย

ผู้แต่ง Satoshi Komoto

เผยแพร่โดย

1 Department of Virology and Parasitology, Fujita Health University School of Medicine, Toyoake, Aichi, Japan, 2 Department of Medical Sciences, National Institute of Health, Nonthaburi, Thailand, 3 Department of Microbiology, Fujita Health University School of Medicine, Toyoake, Aichi, Japan, 4 Department of Pediatrics, Fujita Health University School of Medicine, Toyoake, Aichi, Japan, 5 Department of Disease Control, Ministry of Public Health, Nonthaburi, Thailand

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งานวิจัยที่แนะนำ Full Genome Characterization of Novel DS-1-Like G8P[8] Rotavirus Strains that Have Emerged in Thailand: Reassortment of Bovine and Human Rotavirus Gene Segments in Emerging DS-1-Like Intergenogroup Re Human rotavirus surveillance in hospitalized gastroenteritis cases in Bangkok, Thailand during 2015 - 2018 Characterization of an unusual DS-1-like G8P[8] rotavirus strain from Japan in 2017: evolution of emerging DS-1-like G8P[8] strains through reassortment 10 สุดยอดแนวโน้มเทคโนโลยีเชิงกลยุทธ์สำหรับปี 2020 โดย Gartner The series EP. 4 : Human augmentation Emergence and Characterization of Unusual DS-1-Like G1P[8] Rotavirus Strains in Children with Diarrhea in Thailand Full genome characterization of novel DS-1-like G9P[8] rotavirus strains that have emerged in Thailand High prevalence of equine‐like G3P[8] rotavirus in children and adults with acute gastroenteritis in Thailand Genetic diversity of rotavirus strains circulating in environmental water and bivalve shellfish in Thailand โรคติดเชื้อทางเดินหายใจแบบเฉียบพลันที่มีสาเหตุจากเชื้อ Human Metapneumovirus (hMPV) Characterization of a G10P[14] rotavirus strain from a diarrheic child in Thailand: Evidence for bovine-to-human zoonotic transmission

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ชื่อเรื่อง (EN):	Reassortment of Human and Animal Rotavirus Gene Segments in Emerging DS-1- Like G1P[8] Rotavirus Strains
ผู้แต่ง / หัวหน้าโครงการ (EN):	Satoshi Komoto
บทคัดย่อ (EN):	The emergence and rapid spread of novel DS-1-like G1P[8] human rotaviruses in Japan were recently reported. More recently, such intergenogroup reassortant strains were identified in Thailand, implying the ongoing spread of unusual rotavirus strains in Asia. During rotavirus surveillance in Thailand, three DS-1-like intergenogroup reassortant strains having G3P[8] (RVA/Human-wt/THA/SKT-281/2013/G3P[8] and RVA/Human-wt/THA/SKT- 289/2013/G3P[8]) and G2P[8] (RVA/Human-wt/THA/LS-04/2013/G2P[8]) genotypes were identified in fecal samples from hospitalized children with acute gastroenteritis. In this study, we sequenced and characterized the complete genomes of strains SKT-281, SKT-289, and LS-04. On whole genomic analysis, all three strains exhibited unique genotype constellations including both genogroup 1 and 2 genes: G3-P[8]-I2-R2-C2-M2-A2-N2-T2-E2-H2 for strains SKT-281 and SKT-289, and G2-P[8]-I2-R2-C2-M2-A2-N2-T2-E2-H2 for strain LS-04. Except for the G genotype, the unique genotype constellation of the three strains (P[8]-I2-R2-C2-M2-A2-N2-T2-E2-H2) is commonly shared with DS-1-like G1P[8] strains. On phylogenetic analysis, nine of the 11 genes of strains SKT-281 and SKT-289 (VP4, VP6, VP1-3, NSP1-3, and NSP5) appeared to have originated from DS-1-like G1P[8] strains, while the remaining VP7 and NSP4 genes appeared to be of equine and bovine origin, respectively. Thus, strains SKT-281 and SKT-289 appeared to be reassortant strains as to DS-1-like G1P[8], animal-derived human, and/or animal rotaviruses. On the other hand, seven of the 11 genes of strain LS-04 (VP7, VP6, VP1, VP3, and NSP3-5) appeared to have originated from locally circulating DS-1-like G2P[4] human rotaviruses, while three genes (VP4, VP2, and NSP1) were assumed to be derived from DS-1-like G1P[8] strains. Notably, the remaining NSP2 gene of strain LS-04 appeared to be of bovine origin. Thus, strain LS-04 was assumed to be a multiple reassortment strain as to DS-1-like G1P[8], locally circulating DS-1-like G2P[4], bovine-like human, and/or bovine rotaviruses. Overall, the great genomic diversity among the DS-1-like G1P[8] strains seemed to have been generated through reassortment involving human and animal strains. To our knowledge, this is the first report on whole genome-based characterization of DS-1-like intergenogroup reassortant strains having G3P[8] and G2P[8] genotypes that have emerged in Thailand. Our observations will provide important insights into the evolutionary dynamics of emerging DS-1-like G1P[8] strains and related reassortant ones.
บทคัดย่อ:	ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN):	en
เอกสารแนบ (EN):	http://nih.dmsc.moph.go.th/research/showimgdetil.php?id=485
เผยแพร่โดย (EN):	1 Department of Virology and Parasitology, Fujita Health University School of Medicine, Toyoake, Aichi, Japan, 2 Department of Medical Sciences, National Institute of Health, Nonthaburi, Thailand, 3 Department of Microbiology, Fujita Health University School of Medicine, Toyoake, Aichi, Japan, 4 Department of Pediatrics, Fujita Health University School of Medicine, Toyoake, Aichi, Japan, 5 Department of Disease Control, Ministry of Public Health, Nonthaburi, Thailand
คำสำคัญ (EN):	Reassortment of Human
เจ้าของลิขสิทธิ์ (EN):	National Institute of Health, Department of Medical Science