สืบค้นงานวิจัย
Immunoassay Based on Carbon Nanotubes/Gold Nanoparticles Nanocomposite for Salmonella enterica serovar Typhimurium Detection
Moegiratul Amaro - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Immunoassay Based on Carbon Nanotubes/Gold Nanoparticles Nanocomposite for Salmonella enterica serovar Typhimurium Detection
ผู้แต่ง / หัวหน้าโครงการ (EN): Moegiratul Amaro
บทคัดย่อ (EN): Salmonella enterica serovar Typhimurium is one of the most harmful pathogenic bacteria that leads to outbreak related with foods. Effective, precise, fast, selective and sensitive assay are therefore needed. To improve sensitivity of S. enterica serovar Typhimurium detection, multiwalled carbon nanotubes (MWCNTs) and gold nanoparticles (AuNPs) were combined and used to increase the signal intensity. In this study, immunoassay for S. enterica serovar Typhimurium was developed using a scanometric detection s~stem. MWCNT/AuNP nanocomposite was fabricated by directly assembling of Au + to MWCNTs and allowing the growth of AuNPs on the surface MWCNTs during synthesis process. The MWCNTs/AuNPs nanocomposite was then attached to anti-So typhimurium antibody to be used as a detecting molecule. For the immunoassay system, streptavidin functionalized magnetic beads was used together with MWCNTsIAuNPs and nitrocellulose membrane were used as a solid support. Several parameters such as blocking reagents, sensitivity and selectivity were optimized. Blocking the MWCNTsIAuNPs with 2% BSA and without blocking treatment resulted in high signal intensity. The highest sensitivity was obtained from the reaction without blocking treatment and 2% BSA at 18 and 42 CFU/mL, respectively, at 5 minute silver enhancement. By using MBs/Ab-biotin and MWCNTsIAuNPs complex, when applied to real sample gave low limit of detection as low as 10,914 CFU/mL in plain milk and 134 CFU/mL in low fat milk with 2% BSA blocking. This immunoassay using MBsIAb-biotin and MWCNTsIAuNPs complex provided high selectivity toward S. enterica serovar Typhimurium as it could not detect heat killed E.coli cells in buffer, plain milk and low fat milk.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เผยแพร่โดย (EN): มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าธนบุรี
คำสำคัญ (EN): Gold Nanoparticles
เจ้าของลิขสิทธิ์ (EN): โครงการพัฒนาเครือข่ายระบบห้องสมุดในประเทศไทย
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Immunoassay Based on Carbon Nanotubes/Gold Nanoparticles Nanocomposite for Salmonella enterica serovar Typhimurium Detection
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