คลังข้อมูลการวิจัยการเกษตรไทย

ผู้แต่ง Benjawan Phetsuksiri

เผยแพร่โดย

1 Ministry of Public Health, National Institute of Health, Department of Medical Sciences, Nonthaburi, Thailand 2 Mae Sot Hospital, Department of Medical Technology, Tak, Thailand 3 Hokkaido University, Research Center for Zoonosis Control, Division of Bioresources, Sapporo, Japan 4 Hokkaido University, Global Institution for Collaborative Research and Education, Global Station for Zoonosis Control, Sapporo, Japan

เผยแพร่เมื่อ ไม่ระบุวันที่เผยแพร่

หน่วยงาน ไม่ระบุหน่วยงาน

งานวิจัยที่แนะนำ Comparison of Loop-Mediated Isothermal Amplification, Microscopy, Culture, and PCR for Diagnosis of Pulmonary Tuberculosis Detection of Mycobacterium tuberculosis and Its Rifampicin Resistance by Automated Nucleic Acid Amplification Test เปรียบเทียบการตรวจหาเชื้อวัณโรคแบบรวดเร็ว ด้วยวิธี Xpert MTB/RIF และ TB-LAMP ในผู้ป่วยวัณโรค Comparison of Xpert MTB/RIF and TB-LAMP for rapid detection of Mycobacterium tuberculosis in the Khonkaen H ประสิทธิภาพของวิธี in-house loop-mediated isothermal amplification ในการตรวจหาเชื้อวัณโรคจากตัวอย่างเสมหะ เปรียบเทียบกับวิธี Xpert MTB/RIF Loop-Mediated Isothermal Amplification for Rapid Identification of Mycobacterium tuberculosis in Comparison with Immunochromatographic SD Bioline MPT64 Rapid® in a High Burden Setting Pragmatic accuracy of an in-house loop-mediated isothermal amplification (LAMP) for diagnosis of pulmonary tuberculosis in a Thai community hospital Development of the Rapid Test Kit for the Identification of Campylobacter spp. Based on Loop-mediated Isothermal Amplification (LAMP) in Combination with a Lateral Flow Dipstick (LFD) and Gold Nano-DN การพัฒนาวิธีการตรวจโรคเมลิออยโดสิสด้วย loop-mediated isothermal amplification (LAMP) Development of loop-mediated isothermal amplification (LAMP) for diagnosing melioidosis Development of a rapid, simple method for detecting Naegleria fowleri visually in water samples by Loop-Mediated Isothermal Amplification (LAMP) Development of reverse transcription-loop-mediated isothermal amplification (RT-LAMP) for the detection of the causative agent of covid-19

คัดลอก URL

ชื่อเรื่อง (EN):	The performance of in-house loop-mediated isothermal amplification for rapid detection of Mycobacterium tuberculosis in sputum sample comparing to Xpert MTB/RIF, microscopy and culture
ผู้แต่ง / หัวหน้าโครงการ (EN):	Benjawan Phetsuksiri
บทคัดย่อ (EN):	Simple, low-cost and effective diagnostic tests for tuberculosis (TB) are needed especially in TB-high burden settings. The present study evaluated the performance of an in-house loop-mediated isothermal amplification (LAMP) for diagnosing TB by comparing it to Xpert MTB/RIF, microscopy and culture. In Thailand, a total of 204 excess sputum samples volume after the processing of cultures were used for Mycobacterium tuberculosis (MTB) detection by Xpert MTB/RIF and LAMP. Based on culture results as the gold standard, the overall sensitivity of LAMP and Xpert MTB/RIF were 82.1% (126/153; 95% confidential interval [CI]: 75.4-88.98%) and 86.9 % (133/153; 95% CI: 80.5-90.8%) respectively, and the specificity of both tests was 100% (51/51; 95% CI: 93.0-100.0%). In comparison with Xpert MTB/RIF, the sensitivity and specificity of LAMP were 94.7% (126/133; 95% CI: 89.5-97.9%), and 100.0% (73/73; 95% CI: 94.9-100.0%), respectively. The average threshold cycle (Ct) of Xpert MTB/RIF detection for positive and negative LAMP results was statistically different, of 18.4 and 27.0, respectively (p < 0.05). In comparison with the acid-fast staining technique, and analyzing LAMP and Xpert MTB/RIF in smear-negative/culture-positive specimens, there was an increase of the detection rate by 47.7% (21/44) and 54.6% (24/44). The diagnostic sensitivity and specificity of LAMP appeared to be comparable to those of Xpert MTB/RIF. We claim that this LAMP has potential to provide a sensitive diagnostic test for the rapid TB diagnosis. It allowed a fast detection of MTB before the cultures and it could be used in resource-limited laboratory settings.
บทคัดย่อ:	ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN):	en
เอกสารแนบ (EN):	http://nih.dmsc.moph.go.th/research/showimgdetil.php?id=576
เผยแพร่โดย (EN):	1 Ministry of Public Health, National Institute of Health, Department of Medical Sciences, Nonthaburi, Thailand 2 Mae Sot Hospital, Department of Medical Technology, Tak, Thailand 3 Hokkaido University, Research Center for Zoonosis Control, Division of Bioresources, Sapporo, Japan 4 Hokkaido University, Global Institution for Collaborative Research and Education, Global Station for Zoonosis Control, Sapporo, Japan
คำสำคัญ (EN):	Sputum
เจ้าของลิขสิทธิ์ (EN):	National Institute of Health, Department of Medical Science