สืบค้นงานวิจัย
Comparison of gene expression profiles between human erythroid cells derived from fetal liver and adult peripheral blood
Tangprasittipap A. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Comparison of gene expression profiles between human erythroid cells derived from fetal liver and adult peripheral blood
ผู้แต่ง / หัวหน้าโครงการ (EN): Tangprasittipap A.
บทคัดย่อ (EN): . A key event in human development is the establishment of erythropoietic progenitors in the bone marrow, which is accompanied by a fetal-to-adult switch in hemoglobin expression. Understanding of this event could lead to medical application, notably treatment of sickle cell disease and β-thalassemia. The changes in gene expression of erythropoietic progenitor cells as they migrate from the fetal liver and colonize the bone marrow are still rather poorly understood, as primary fetal liver (FL) tissues are difficult to obtain. Methods. We obtained human FL tissue and adult peripheral blood (AB) samples from Thai subjects. Primary CD34 + cells were cultured in vitro in a fetal bovine serumbased culture medium. After 8 days of culture, erythroid cell populations were isolated by flow cytometry. Gene expression in the FL- and AB-derived cells was studied by Affymetrix microarray and reverse-transcription quantitative PCR. The microarray data were combined with that from a previous study of human FL and AB erythroid development, and meta-analysis was performed on the combined dataset. Results. FL erythroid cells showed enhanced proliferation and elevated fetal hemoglobin relative to AB cells. A total of 1,391 fetal up-regulated and 329 adult up-regulated genes were identified from microarray data generated in this study. Five hundred ninety-nine fetal up-regulated and 284 adult up-regulated genes with reproducible patterns between this and a previous study were identified by metaanalysis of the combined dataset, which constitute a core set of genes differentially expressed between FL and AB erythroid cells. In addition to these core genes, 826 and 48 novel genes were identified only from data generated in this study to be FL up- and AB up-regulated, respectively. The in vivo relevance for some of these novel genes was demonstrated by pathway analysis, which showed novel genes functioning in pathways known to be important in proliferation and erythropoiesis, including the mitogenactivated protein kinase (MAPK) and the phosphatidyl inositol 3 kinase (PI3K)-Akt pathways. Discussion. The genes with upregulated expression in FL cells, which include many novel genes identified from data generated in this study, suggest that cellular proliferation pathways are more active in the fetal stage. Erythroid progenitor cells may thus undergo a reprogramming during ontogenesis in which proliferation is modulated by changes in expression of key regulators, primarily MYC, and others including insulinlike growth factor 2 mRNA-binding protein 3 (IGF2BP3), neuropilin and tolloid-like 2 (NETO2), branched chain amino acid transaminase 1 (BCAT1), tenascin XB (TNXB) and proto-oncogene, AP-1 transcription factor subunit (JUND). This reprogramming may thus be necessary for acquisition of the adult identity and switching of hemoglobin expression. © 2018 Tangprasittipap et al.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-85052687694&doi=10.7717%2fpeerj.5527&partnerID=40&md5=bf21165139fb911495f5b84790d93170
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): upregulation
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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Tangprasittipap A.. "Comparison of gene expression profiles between human erythroid cells derived from fetal liver and adult peripheral blood". ไม่ระบุหน่วยงาน. ม.ป.ป.

Tangprasittipap A.. "Comparison of gene expression profiles between human erythroid cells derived from fetal liver and adult peripheral blood". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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Comparison of gene expression profiles between human erythroid cells derived from fetal liver and adult peripheral blood
Tangprasittipap A.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
Qualitative and quantitative comparison of the proteome of erythroid cells differentiated from human iPSCs and adult erythroid cells by multiplex TMT labelling and nanoLC-MS/MS Excretory-secretory product of third-stage Gnathostoma spinigerum larvae induces apoptosis in human peripheral blood mononuclear cells In vitro production of functional immune cells derived from human hematopoietic stem cells Downregulation of transcription factor LRF/ZBTB7A increases fetal hemoglobin expression in -thalassemia/hemoglobin E erythroid cells UNC0638 induces high levels of fetal hemoglobin expression in β-thalassemia/HbE erythroid progenitor cells CCAAT-enhancer binding protein-α (C/EBPα) and hepatocyte nuclear factor 4α (HNF4α) regulate expression of the human fructose-1,6-bisphosphatase 1 (FBP1) gene in human hepatocellular carcinoma HepG2 ce Blood-induced differential gene expression in Anopheles dirus evaluated using RNA sequencing รูปแบบดีเอ็นเอเมทิลเลชั่นของเซลล์ต้นกำเนิดเม็ดเลือดแดงทารกในครรภ์และผู้ใหญ่ DNA Methylation Profiles In Human Fetal And Adult Red Blood Cell Progenitor Identification of IL-1α gene from bovine peripheral blood mononuclear cells by polymerase chain reaction (Buffalo Bulletin (Thailand),36(2)) Vitellogenin gene expression at different ovarian stages in the giant freshwater prawn, Macrobrachium rosenbergii, and stimulation by 4-nonylphenol
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