สืบค้นงานวิจัย
In vitro production of functional immune cells derived from human hematopoietic stem cells
Payuhakrit W. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): In vitro production of functional immune cells derived from human hematopoietic stem cells
ผู้แต่ง / หัวหน้าโครงการ (EN): Payuhakrit W.
บทคัดย่อ (EN): Hematopoietic stem cells (HSC) from cord blood are potentially high sources for transplantation due to their low immunogenicity and the presence of the multipotent cells. These cells are capable of differentiating to produce various lineages of blood cells under specific conditions. We have enriched highly purified CD34+ cells from cord blood, determined in vitro growth of the cells in culture systems in the absence (condition A) or presence of GM-CSF and G-CSF (condition B), and determined the profile of immune cells during the period of cultivation by using flow cytometry. PhytohemagglutininA (PHA) was used as a mitogen to stimulate T lymphocytes derived from hematopoietic stem cells. GM-CSF and G-CSF prolonged the survival of the growing cells and also maintained expansion of cells in blastic stage. By day 12 of cultivation, when cell numbers peaked, various types of immune cells had appeared (CD14+ cells, CD40+HLA-DR+ cells, CD3+CD56+ cells, CD19+ cells, CD3+CD4+ cells, CD3+CD8+cells and CD3-CD56+). A significantly higher percentage of monocytes (p = 0.002) were observed under culture with GM-CSF, G-CSF when compared with culture without GM-CSF, G-CSF. In addition, T lymphocytes derived from HSC responded to 50 μg/ml of PHA. This is the first report showing the complete differentiation and proliferation of immune cells derived from CD34+ HSC under in vitro culture conditions. Lymphocytes, monocytes, dendritic cells and polymorph nuclear cells derived from HSC in vitro are unique, and thus may benefit various studies such as innate immunity and pathophysiology of immune disorders. © 2015, Leibniz Research Centre for Working Environment and Human Factors. All right reserved.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-84941348635&doi=10.17179%2fexcli2015-506&partnerID=40&md5=77eaeaba329962ee5ed922b888743661
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): umbilical cord blood
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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Payuhakrit W.. "In vitro production of functional immune cells derived from human hematopoietic stem cells". ไม่ระบุหน่วยงาน. ม.ป.ป.

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In vitro production of functional immune cells derived from human hematopoietic stem cells
Payuhakrit W.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
Generation of porcine induced-pluripotent stem cells from Sertoli cells Rabbit induced pluripotent stem cells retain capability of in vitro cardiac differentiation Comparison of gene expression profiles between human erythroid cells derived from fetal liver and adult peripheral blood Biology of Zika virus infection in human skin cells Antisense oligonucleotide induction of progerin in human myogenic cells A time course analysis of the electrophysiological properties of neurons differentiated from human induced Pluripotent Stem Cells (iPSCs) Dual small-molecule targeting of SMAD signaling stimulates human induced pluripotent stem cells toward neural lineages Qualitative and quantitative comparison of the proteome of erythroid cells differentiated from human iPSCs and adult erythroid cells by multiplex TMT labelling and nanoLC-MS/MS Effect of 8-hydroxyquinoline and derivatives on human neuroblastoma SH-SY5Y cells under high glucose Computational identification of miRNAs that modulate the differentiation of mesenchymal stem cells to osteoblasts
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