สืบค้นงานวิจัย
DEVELOPMENT OF GYNOGENIC HAPLOIDS THROUGH EMBRYO SAC CULTURE IN HEVEA BRASILIENSIS
Divya U.K. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): DEVELOPMENT OF GYNOGENIC HAPLOIDS THROUGH EMBRYO SAC CULTURE IN HEVEA BRASILIENSIS
ผู้แต่ง / หัวหน้าโครงการ (EN): Divya U.K.
บทคัดย่อ (EN): Hevea brasiliensis, belonging to the family Euphorbiaceae, is cultivated mainly for its latex, which is the sole commercial source of Natural Rubber (NR). Haploids are of great significance in plant breeding. They find many applications in various basic plant research disciplines like traditional plant breeding, plant biotechnology, cytogenetics and genomic studies including gene mapping. Development of haploids/ homozygous diploids poses a major challenge in Hevea breeding. Conventional techniques for the production of homozygous plants require several backcrosses, thus rendering the whole process quite lengthy and tedious in the case of a perennial tree crop like Hevea with a long breeding cycle. In vitro development of haploids is a viable alternate approach to conventional breeding. Presence of chromosomes in single copy in gametes (n) and the concept of totipotency together have led to the in-vitro development of haploids in many plant species. This paper describes optimization of protocols for the isolation and culture of intact embryo sacs in Havea clone RRII 414 for the generation of gynogenic haploids. Ovules dissected out from mature female flowers prior to anthesis were used as the source of embryo sacs. Pretreatment of the ovules in two different nutrient media (MS and K&M) enriched with various levels of sucrose (2-15%) for different time intervals (2-14 days) was attempted for the successful isolation of intact and viable embryo sacs in good numbers. Pretreatment was carried out using both solid and liquid media. From the pretreated ovules, embryo sacs were isolated mechanically and cultured into different basal media (modified MS, K&M, WPM and Nitsch) fortified with combinations of the growth regulators 2,4-D, BA & Kinetin (0.92 -9.2 µM). Proliferated callus was transferred to modified MS fortified with different concentrations of GA (0.57 µM-2.8 µM) and BA (0.92-9.2 µM) for embryo induction. Pretreatment of ovules for 8-10 day in liquid MS medium containing 10% sucrose was found to be most ideal for isolation of intact embryo sacs. Callus initiation was observed from the embryo sacs cultured in K&M medium supplemented with 4.6 µM 2,4-D and 1.8 µM Kinetin. Emergence of embryogenic callus followed by the development of globular proembryos was observed in the presence of BA (7.3 µM) and GA (1.7 µM). cytological analysis of the callus was carried out using the standard procedure and a chromosome count of n=18 was obtained thereby confirming haploidy. These calli/ embryos can be used for the development of haploids and homozygous diploids in Hevea. This is the first report of development of gynogenic haploids and ploidy confirmation in Hevea brasiliensis.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เผยแพร่โดย (EN): การยางแห่งประเทศไทย
คำสำคัญ (EN): growth regulators
เจ้าของลิขสิทธิ์ (EN): การยางแห่งประเทศไทย
หากไม่พบเอกสารฉบับเต็ม (Full Text) โปรดติดต่อหน่วยงานเจ้าของข้อมูล

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Divya U.K.. "DEVELOPMENT OF GYNOGENIC HAPLOIDS THROUGH EMBRYO SAC CULTURE IN HEVEA BRASILIENSIS". ไม่ระบุหน่วยงาน. ม.ป.ป.

Divya U.K.. "DEVELOPMENT OF GYNOGENIC HAPLOIDS THROUGH EMBRYO SAC CULTURE IN HEVEA BRASILIENSIS". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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DEVELOPMENT OF GYNOGENIC HAPLOIDS THROUGH EMBRYO SAC CULTURE IN HEVEA BRASILIENSIS
Divya U.K.
การยางแห่งประเทศไทย
ไม่ระบุวันที่เผยแพร่
Plant Hormones and Oxidative Stress in Hevea brasiliensis Localization of Peroxidase Enzyme in the Bark of Hevea brasiliensis Effect of Temperature Constraints on Photosynthesis of Rubber (Hevea brasiliensis) Association of a Viroid with Tapping Panel Dryness of Hevea brasiliensis and its Molecular Characterization Drought Tolerance in Transgenic MsSOD Hevea brasiliensis in A Dry Humid Environment Cloning and expression of 3-hydroxy-3-methylglutaryl-Coa Reductase 1 (Hmge 1) from Hevea brasiliensis Agrobacterium Strain- A Key Factor Determining the Efficiency of Transformation in Hevea brasiliensis DEVELOPMENT OF DWARF CLONES OF HEVEA BRASILIENSIS Evaluation of some clones (Hevea brasiliensis) in a small scale trial in the southern part of Guatemala Effects of drought and tapping for latex production on water relations of Hevea brasiliensis trees
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