คลังข้อมูลการวิจัยการเกษตรไทย

ผู้แต่ง Sumalee Panthong

เผยแพร่โดย

2 Department of Applied Thai Traditional Medicine, Faculty of Medicine, Thammasat University, Pathumthani 12120, Thailand 3 National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand, 4 Center of Excellence in Applied Thai Traditional Medicine Research (CEATMR), Thammasat University, Pathumthani 12120, Thailand

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หน่วยงาน ไม่ระบุหน่วยงาน

งานวิจัยที่แนะนำ ปริมาณ dioscorealide B ของยอดข้าวเย็นใต้ (Dioscorea membranacea Pierre ex Prain & Burkill) ในสภาพปลอดเชื้อที่ระยะเวลาเพาะเลี้ยงต่างกัน Biological activity of dolichandrone serrulata flowers and their active components Neuraminidase activity and resistance of 2009 pandemic H1N1 influenza virus to antiviral activity in bronchoalveolar fluid Cytotoxic sesquiterpenoids and diarylheptanoids from the rhizomes of Curcuma elata Roxb. Antihyperglycemic activity of Ophiocordyceps sinensis: A Review Fingerroot, Boesenbergia rotunda and its aphrodisiac activity Pea, Pisum sativum, and its anticancer activity คุณสมบัติทางเคมีกายภาพและโครงสร้างโมเลกุลของอะไมโลเพคตินในแป้งกลอย (Dioscorea hispida Dennst.) และแป้งมันมือเสือ (Dioscorea esculenta (Lour.) Burkill) (ระยะที่ 2) Antithrombotic activity of turmeric (Curcuma longa): A review Anti-HIV activity of alkaloids from dasymaschalon echinatum

คัดลอก URL

ชื่อเรื่อง (EN):	Immunomodulatory activity of Dioscorea membranacea Pierre rhizomes and of its main active constituent Dioscorealide B
ผู้แต่ง / หัวหน้าโครงการ (EN):	Sumalee Panthong
บทคัดย่อ (EN):	Background: The rhizomes of Dioscorea membranacea Pierre, also called Hua-Khao-Yen by Thai name, are used as ingredients in many Thai traditional medicines for the alternative or complementary treatment of cancer and AIDs. Preliminary in vitro studies have indicated that D. membranacea extracts exhibited high cytotoxic activity with several cancer cell lines, but the underlining mechanisms are far from clear. The aims of this study were to investigate the effects of ethanolic and aqueous crude extracts from D. membranacea Pierre, and pure compound from D. membranacea Pierre, Dioscorealide B, on natural killer cells activity and on lymphocyte proliferation. Methods: Immunomodulatory activity was investigated using PBMCs from healthy donors. NK cells activity was performed by the chromium release assay using PBMCs as effector cells, and K562 cells line labelled with chromium as target cells. Lymphocyte proliferation was determined by 3H-thymidine uptake. The degree of activation was expressed as the stimulation index. Results: The crude ethanolic extracts of D. membranacea Pierre significantly stimulated NK cells activity against K562 cells line at lower concentrations of 10 and 100 ng/ml, but not at higher concentrations. The ethanolic extracts showed no observable effect on lymphocyte proliferation. The crude water extracts significantly increased NK cell activity at concentrations of 10 ng/ml, 100 ng/ml, 1 μg/ml, 10 μg/ml and 100 μg/ml, and also activated lymphocyte proliferation at concentration of 1 ng/ml, 10 ng/ml, 100 ng/ml, 1 μg/ml, 5 μg/ml, 10 μg/ml and 100 μg/ml. However, Dioscorealide B had no significant effect at lower concentrations (0–1 μg/ml and 0–0.1 μg/ml, respectively) on NK cell activity and lymphocyte proliferation. In fact higher concentrations (>10 μg/ml and >0.5 μg/ml) of Dioscorealide B cause a significant decrease in NK cell activity and lymphocyte proliferation. Conclusions: D. membranacea Pierre stimulated NK cells activity and lymphocyte proliferation, but Dioscorealide B either had no effect, and at higher concentrations decreased NK cell activity and lymphocyte proliferation. Our results suggest that both extracts of D. membranacea Pierre significantly increases immune function, but the underlining mechanism is not clearly understood.
บทคัดย่อ:	ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN):	en
เอกสารแนบ (EN):	http://nih.dmsc.moph.go.th/research/showimgdetil.php?id=466
เผยแพร่โดย (EN):	2 Department of Applied Thai Traditional Medicine, Faculty of Medicine, Thammasat University, Pathumthani 12120, Thailand 3 National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand, 4 Center of Excellence in Applied Thai Traditional Medicine Research (CEATMR), Thammasat University, Pathumthani 12120, Thailand
คำสำคัญ (EN):	Dioscorealide B
เจ้าของลิขสิทธิ์ (EN):	National Institute of Health, Department of Medical Science