สืบค้นงานวิจัย
L-carnitine supplemented extender improves cryopreserved-thawed cat epididymal sperm motility
Manee-In S. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): L-carnitine supplemented extender improves cryopreserved-thawed cat epididymal sperm motility
ผู้แต่ง / หัวหน้าโครงการ (EN): Manee-In S.
บทคัดย่อ (EN): Cryopreservation of epididymal sperm is an effective technique to preserve genetic materials of domestic cats and wild felids when they unexpectedly die. However, this technique inevitably causes detrimental changes of cryopreserved-thawed spermatozoa, for example, by physical damage and excessive oxidative stress. L-carnitine is an antioxidant that has been used to improve sperm motility in humans and domestic animals. This study aimed to investigate the effects of L-carnitine on cat epididymal sperm quality following cryopreservation and thawing. After routine castration, cauda epididymides were collected from 60 cat testes. The epididymal spermatozoa from 3 cauda epididymides were pooled as 1 replicate. Spermatozoa samples (16 replicates) were examined for spermatozoa quality and then randomly divided into 4 groups: 0 mM L-carnitine (control), 12.5 mM, 25 mM and 50 mM L-carnitine. The sperm aliquots were then equilibrated and conventionally frozen. After thawing, sperm motility, plasma membrane integrity, DNA integrity and acrosome integrity were evaluated. The 25 mM L-carnitine significantly improved sperm motility compared with a control group (p<0.05), although this was not significantly different among other concentrations. In conclusion, supplementation of 25 mM L-carnitine in freezing extender improves cauda epididymal spermatozoa motility. The effects of L-carnitine on the levels of oxidative stress during freezing and thawing remains to be examined. © 2014 by Asian-Australasian Journal of Animal Sciences.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-84899543136&doi=10.5713%2fajas.2013.13565&partnerID=40&md5=dc1fab301bdc67e70efd0d46b98575d4
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): L-carnitine
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
หากไม่พบเอกสารฉบับเต็ม (Full Text) โปรดติดต่อหน่วยงานเจ้าของข้อมูล

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Manee-In S.. "L-carnitine supplemented extender improves cryopreserved-thawed cat epididymal sperm motility". ไม่ระบุหน่วยงาน. ม.ป.ป.

Manee-In S.. "L-carnitine supplemented extender improves cryopreserved-thawed cat epididymal sperm motility". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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L-carnitine supplemented extender improves cryopreserved-thawed cat epididymal sperm motility
Manee-In S.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
Effect of extracellular adenosine 5'-triphosphate on cryopreserved epididymal cat sperm intracellular ATP concentration, sperm quality, and in vitro fertilizing ability Low-density lipoprotein improves motility and plasma membrane integrity of cryopreserved canine epididymal spermatozoa Cryopreservation and storage of cat epididymal sperm using ‒75 °C freezer vs liquid nitrogen Determination of appropriate cryopreservation protocols for epididymal cat spermatozoa Frozen-thawed quality of swamp buffalo spermatozoa cryopreserved with sugar palm juice extender (Buffalo Bulletin,39(3)) Cholesterol supplementation in extender improves quality of frozen-thawed Nili-Ravi buffalo bull semen (Buffalo Bulletin (Thailand),35(2)) Reproductive aging in male dogs: The epididymal sperm defects and expression of androgen receptor in reproductive tissues Effects of Addition of Reduced Glutathione to Thawing media on Motility Parameters, Lipid Peroxidation and Fertility Rate in Frozen-Thawed Chicken Spermatozoa Changes of fatty acids in phosphatidylcholine on sperm membrane during Macrobrachium rosenbergii sperm transit through spermatic duct and lipid analysis in spermatic vesicles Protocols for sperm cryopreservation in the domestic cat: A review
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