คลังข้อมูลการวิจัยการเกษตรไทย

ผู้แต่ง Sittiporn Parnmen

เผยแพร่โดย

1 Toxicology Center, National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi 11000, Thailand 2 Microbe Interaction Laboratory, BIOTEC, National Science and Technology Development Agency, Pathum Thani 12120, Thailand 3 Department of Biology, Faculty of Science, Srinakharinwirot University, Bangkok 10110, Thailand 4 Department of Biology & Natural Medicinal Mushroom Museum, Faculty of Science, Mahasarakham University, Mahasarakham 44150, Thailand

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งานวิจัยที่แนะนำ Group B Streptococcal bacteremia burden and trends over time, 2007-15, Thailand Full genome characterization of novel DS-1-like G9P[8] rotavirus strains that have emerged in Thailand Wolbachia supergroups A and B in natural populations of medically important filth flies (Diptera : Muscidae, Calliphoridae, and Sarcophagidae) in Thailand Prevalence of zoonotic Bartonella species among rodents and shrews in Thailand Draft Genome Sequence of a Clostridium botulinum Isolate from Thailand Harboring the Subtype bont/B8 Gene Circulation of HIV-1 Multiple Complexity Recombinant Forms Among Female Sex Workers Recently Infected with HIV-1 in Thailand Molecular Characterization of Clostridium botulinum Isolates from Foodborne Outbreaks in Thailand, 2010 Mangrove forests in Thailand Progress on Rubber Breeding of Thailand DNA barcoding for identification of poisonous mushrooms from 187 cases of mycetism in Thailand

คัดลอก URL

ชื่อเรื่อง (EN):	Molecular identification of poisonous mushrooms using nuclear ITS region and peptide toxins: a retrospective study on fatal cases in Thailand
ผู้แต่ง / หัวหน้าโครงการ (EN):	Sittiporn Parnmen
บทคัดย่อ (EN):	Cases of mushroom poisoning in Thailand have increased annually. During 2008 to 2014, the cases reported to the National Institute of Health included 57 deaths; at least 15 died after ingestion of amanitas, the most common lethal wild mushrooms inhabited. Hence, the aims of this study were to identify mushroom samples from nine clinically reported cases during the 7-year study period based on nuclear ITS sequence data and diagnose lethal peptide toxins using a reversed phase LC-MS method. Nucleotide similarity was identified using BLAST search of the NCBI database and the Barcode of Life Database (BOLD). Clade characterization was performed by maximum likelihood and Bayesian phylogenetic approaches. Based on BLAST and BOLD reference databases our results yielded high nucleotide similarities of poisonous mushroom samples to A. exitialis and A. fuliginea. Detailed phylogenetic analyses showed that all mushroom samples fall into their current classification. Detection of the peptide toxins revealed the presence of amatoxins and phallotoxins in A. exitialis and A. fuliginea. In addition,toxic α-amanitin was identified in a new provisional species, Amanita sp.1, with the highest toxin quantity. Molecular identification confirmed that the mushrooms ingested by the patients were members of the lethal amanitas in the sections Amanita and Phalloideae. In Thailand, the presence of A. exitialis was reported here for the first time and all three poisonous mushroom species provided new and informative data for clinical studies.
บทคัดย่อ:	ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN):	en
เอกสารแนบ (EN):	http://nih.dmsc.moph.go.th/research/showimgdetil.php?id=494
เผยแพร่โดย (EN):	1 Toxicology Center, National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi 11000, Thailand 2 Microbe Interaction Laboratory, BIOTEC, National Science and Technology Development Agency, Pathum Thani 12120, Thailand 3 Department of Biology, Faculty of Science, Srinakharinwirot University, Bangkok 10110, Thailand 4 Department of Biology & Natural Medicinal Mushroom Museum, Faculty of Science, Mahasarakham University, Mahasarakham 44150, Thailand
คำสำคัญ (EN):	Phallotoxin
เจ้าของลิขสิทธิ์ (EN):	National Institute of Health, Department of Medical Science