สืบค้นงานวิจัย
Synthesis of Silvernanoparticles Using Hevea Leaf and Latex
Jayasree Gopalakrishnan - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Synthesis of Silvernanoparticles Using Hevea Leaf and Latex
ผู้แต่ง / หัวหน้าโครงการ (EN): Jayasree Gopalakrishnan
บทคัดย่อ (EN): Nanoparticles are the fundamental building blocks of nanotechnology. The most effectively studied nanoparticles are made from noble metals such as Silver, Platinum, Gold and Palladium. These nanoparticles have applications in the area of catalysis, optoelectronic, diagnostic biological probes and display devices. Among the noble metals mentioned, silver nanoparticles (AgNPs) play significant role in the field of biology and medicine. A number of chemical and physical methods are available for its synthesis. In this respect nanoparticle synthesis using plants can be advantageous over other biological processes because it eliminates the elaborate process of maintaining cell cultures and can also be suitably scaled up for large-scale synthesis of nanoparticles under non-aseptic environment. Already scientists have started utilizing the bio-based synthesis of nanomaterials using leaf extracts and microorganisms. Synthesis utilizing plants/ parts of plants was advantageous and often found to be kinetically favourable than other bio-processes. A number of plant species has been successfully used for synthesis of silver nanoparticles. The present study was conducted to evaluate the unexploited plant sources in the development of silver nanoparticles. Hevea leaf extract and latex was used and their rate of reduction of silver nitrate was investigated. Fresh and healthy Hevea leaves were collected, washed thoroughly with distilled water and cut in to small pieces. About 5g of this finely cut Hevea leaves were weighed and transferred in to 250 ml Erlnmeyer flask containing 100 ml milli Q water, mixed well and boiled for 5 minute. After cooling, the extract obtained was filtered through Whatman No.1 filter paper and the filtrate was collected in 100ml Erlnmeyer flask and stored in refrigerator. Aqueous silver nitrate (AgNO3) solution (2 mM) was prepared for the synthesis of silver nanoparticles from Hevea leaf extract. 0.1ml of 0.1 N NaOH was added to the leaf extract. 5ml of Hevea leaf extract was added to 20ml of aqueous solution of 2 mM AgNO3 for reduction in to silver ions. Similarly about 50ml of latex was taken in a100 ml beaker into this 15ml 3%acetic acid was added and the extract was collected and stored. Aqueous solution of AgNO3 (10 mM) was prepared for the synthesis of silver nanoparticles from latex extract. 5 ml of latex extract was added to 20 ml of aqueous solution of 10 mM AgNO3 for reduction in to silver ions. These reaction mixtures were heated in a water bath at 85oC for 30 minute. The reduced silver nanoparticles were characterized using UV-VIS, X – ray diffraction (XRD) and Scanning electron microscopy (SEM). The qualitative analysis for nitrate reduction and the antimicrobial activity of the synthesized nanoparticles was also performed. It was found that the leaf extract and latex could be able to reduce silver ion and produce AgNPs. Visually this was identified by the gradual clour change of the reaction mixture. Formation of silver nanoparticle was evaluated through spectrophotometer in a range of wavelength from 300 to 600 nm. The reaction mixture exhibited strong absorption at 390 and 420 nm and broadening of peak indicated that the particles are polydispersed. The silver nanostructure synthesized using bio-extract from Hevea leaf and latex was further demonstrated and confirmed by XRD. The XRD pattern showed intense peaks in the whole of spectrum of 2θ value ranging from 20 to 80. Peaks observed at 390, 65.30, 780 angle in the case of leaf extract and in the case of latex it was at 38.80, 65.190, 78.360. This confirms the crystalline nature of nanoparticles synthesized. SEM analysis reveals the structural view of the silver nanoparticles. The SEM images show the high density nanostructures synthesized by Hevea leaf extract and latex and the size of nanoparticle was less than 80nm. This is further confirmed the existance of AgNPs. The antimicrobial activity is well demonstrated by considerable zone of inhibition against E.coli compared with the standard drug ampicillin. The biological synthesis of AgNPs using Hevea brasiliensis leaf and latex extract provides an environmental friendly, simple and efficient route for synthesis of nanoparticles. It proved to have excellent antimicrobial activity. Morphology and size of nanoparticles were strongly depending on the process parameters. Fine tuning of the process parameters may give product with typical characteristics. Therefore a detailed study is essential for parameters setting, characterizing the reductants and stabilizing compounds from Hevea leaf and latex
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เผยแพร่โดย (EN): การยางแห่งประเทศไทย
คำสำคัญ (EN): stabilizing compounds
เจ้าของลิขสิทธิ์ (EN): การยางแห่งประเทศไทย
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Jayasree Gopalakrishnan. "Synthesis of Silvernanoparticles Using Hevea Leaf and Latex". ไม่ระบุหน่วยงาน. ม.ป.ป.

Jayasree Gopalakrishnan. "Synthesis of Silvernanoparticles Using Hevea Leaf and Latex". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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Synthesis of Silvernanoparticles Using Hevea Leaf and Latex
Jayasree Gopalakrishnan
การยางแห่งประเทศไทย
ไม่ระบุวันที่เผยแพร่
Insights of Hevea Latex Serum Proteins Using Shotgun Proteomics Analysis IMPROVEMENT AND APPLICATION OF THE TECHNIQUE OF MINI-SEEDLING BUDDING OF HEVEA BRASILIENSIS Hevea B Serum Proteome Profiling Using Liquid Chromatography-Mass Spectrometry Generation of Tranceriptome Recources in Rubber (Hevea brasiliensis) in Response to Corynespora Cassiicola Causing Corynespora Leaf Disease for Gene Discovery and Marker Identification Using Ngs Platf Lipid composition of Hevea brasiliensis latex and dry rubber: characterization and relation with some physical properties A MODIFIED STIMULATION METHOD IN HEVEA BRASILIENSIS FOR REDUCING OXIDATIVE STRESS ASSESSMENT OF RUBBER LATEX PRODUCTION BASED ON DIAMETER CLASS AND FREQUENCY OF TAPPING AND COLLECTION FOR SUSTAINABLE LATEX PRODUCTION AND MANAGEMENT IN THE PHILIPPINES The source of latex. Tracing carbon from leaf photosynthesis to latex metabolism in rubber trees using carbon stable isotopes PROGRESS OF RRIT’s HEVEA SOMATIC EMBRYOGENESIS Plant Hormones and Oxidative Stress in Hevea brasiliensis
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