สืบค้นงานวิจัย
Biochemical characterization of a new glycosylated protease from Euphorbia cf. lactea latex
Siritapetawee J. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Biochemical characterization of a new glycosylated protease from Euphorbia cf. lactea latex
ผู้แต่ง / หัวหน้าโครงการ (EN): Siritapetawee J.
บทคัดย่อ (EN): A dimeric protease designated as EuP-82 was purified from Euphorbia cf. lactea latex. Since its proteolytic activity was inhibited by a serine protease specific inhibitor (PMSF), EuP-82 was classified as a serine protease. N-glycan deglycosylation tests revealed that EuP-82 was a glycosylated protein. MALDI-TOF MS showed that EuP-82 was a homodimer, which was its active form. The optimal conditions for fibrinogenolytic activity were at pH 11 and 35°C. EuP-82 enzyme had broad range of pH stability from 4 to 12. Moreover, the enzyme was still active in the presence of reducing agent (β-mercaptoethanol). EuP-82 was a proline-rich enzyme (about 20.69mol%). Increased proline production can be found in higher plants in response to both biotic and abiotic stresses, high proline in the molecule of EuP-82 might stabilize its activity, structure and folding. Based on the N-terminal amino acid sequences and peptide mass fingerprint (PMF) of EuP-82, the enzyme was identified as a new serine protease. The digested products from EuP-82 cleavage of human fibrinogen were analyzed by SDS-PAGE and PMF. The results confirmed that EuP-82 could digest all subunits of human fibrinogen. EuP-82 cleaved fibrinogen with a Michaelis constant (Km) of 3.30 ± 0.26 μM; a maximal velocity (Vmax) of 400.9 ± 0.85 ng min-1; and a catalytic efficiency (Vmax/Km) of 121.5 ± 9.25 ng μM-1min-1. EuP-82 has potential for use in medicinal treatment, for example thrombosis, since the enzyme had fibrinogenolytic activity and high stability. © 2015 Elsevier Masson SAS.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-84927733107&doi=10.1016%2fj.plaphy.2015.04.012&partnerID=40&md5=8e7e8c112bef6e217ed8f6a513c71cde
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Thrombosis
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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Siritapetawee J.. "Biochemical characterization of a new glycosylated protease from Euphorbia cf. lactea latex". ไม่ระบุหน่วยงาน. ม.ป.ป.

Siritapetawee J.. "Biochemical characterization of a new glycosylated protease from Euphorbia cf. lactea latex". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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Biochemical characterization of a new glycosylated protease from Euphorbia cf. lactea latex
Siritapetawee J.
มหาวิทยาลัยมหิดล
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