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Development and application of a loop-mediated isothermal amplification method for rapid detection of Pasteurella multocida (Buffalo Bulletin,41(2))
Narinder Singh Sharma - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Development and application of a loop-mediated isothermal amplification method for rapid detection of Pasteurella multocida (Buffalo Bulletin,41(2))
ผู้แต่ง / หัวหน้าโครงการ (EN): Narinder Singh Sharma
หน่วยงานสังกัดผู้แต่ง (EN): Punjab (India). Department of Veterinary Microbiology
บทคัดย่อ (EN): Pasteurella multocida is an important pathogen affecting livestock and poultry causing significant economic losses. A loop-mediated isothermal amplification (LAMP) assay using four primers targeting a conserved region of the kmt1 gene was standardised to diagnose Pasteurella multocida. The test was carried out at 64 deg C for 45 minutes, the LAMP products could be visually confirmed using fluorescent dye SYBR Green I as detection reagent both with naked eye as well as under UV-illumination. The sensitivity of the developed LAMP assay was 10**(4) fold higher than PCR. Furthermore, no cross-reactivity was found with the other tested bacteria. The developed LAMP assay allows easy, rapid, accurate and sensitive detection of Pasteurella multocida.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://kukr.lib.ku.ac.th/db/kukr/search_detail/result/418074
คำสำคัญ (EN): LAMP
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Development and application of a loop-mediated isothermal amplification method for rapid detection of Pasteurella multocida (Buffalo Bulletin,41(2))
Narinder Singh Sharma
ไม่ระบุผู้เผยแพร่
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Isolation and molecular detection of biofilm producing and multiple drug resistant Enterococcus faecalis from the buffalo meat (Buffalo Bulletin,41(3)) Molecular characterization of buffalo alpha s1-casein gene (Buffalo Bulletin,41(3)) Hydrallantois in a graded Murrah buffalo - A case report (Buffalo Bulletin,41(2)) Fatty acid profile of Murrah buffalo milk fat (Buffalo Bulletin,41(1)) Genetic parameters prediction for Khuzestani buffaloes milk characteristics by random regression method (Buffalo Bulletin,41(1)) Constraints of buffalo farming in Kallakurichi district of Tamil Nadu, India (Buffalo Bulletin,41(3)) Dystocia due to bull dog calf in a Murrah buffalo heifer (Buffalo Bulletin,41(1)) Dystocia due to Dicephalic dipus dibrachius monster in a Murrah buffalo (Buffalo Bulletin,41(3)) Expression and structural variations of toll-like receptor 4 gene in buffalo and cattle (Buffalo Bulletin,41(1)) Quality evaluation and safety assessment of buffalo milk collected from Mathura city (Buffalo Bulletin,41(3))
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