สืบค้นงานวิจัย
Pesticide analysis using nanoceria-coated paper-based devices as a detection platform
Nouanthavong S. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Pesticide analysis using nanoceria-coated paper-based devices as a detection platform
ผู้แต่ง / หัวหน้าโครงการ (EN): Nouanthavong S.
บทคัดย่อ (EN): We report the first use of a paper-based device coated with nanoceria as a simple, low-cost and rapid detection platform for the analysis of organophosphate (OP) pesticides using an enzyme inhibition assay with acetylcholinesterase (AChE) and choline oxidase (ChOX). In the presence of acetylcholine, AChE and ChOX catalyze the formation of H2O2, which is detected colorimetrically by a nanoceria-coated device resulting in the formation of a yellow color. After incubation with OP pesticides, the AChE activity was inhibited, producing less H2O2, and a reduction in the yellow intensity. The assay is able to analyze OP pesticides without the use of sophisticated instruments and gives detection limits of 18 ng mL-1 and 5.3 ng mL-1 for methyl-paraoxon and chlorpyrifos-oxon, respectively. The developed method was successfully applied to detect methyl-paraoxon in spiked vegetables (cabbage) and a dried seafood product (dried green mussel), obtaining ∼95% recovery values for both sample types. The spiked samples were also analyzed using LC-MS/MS as a comparison to the developed method and similar values were obtained, indicating that the developed method gives accurate results and is suitable for OP analysis in real samples. © 2016 The Royal Society of Chemistry.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-84959450427&doi=10.1039%2fc5an02403j&partnerID=40&md5=e9f311951c74b3dc8497c5c51a08c9bf
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Pesticides
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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Pesticide analysis using nanoceria-coated paper-based devices as a detection platform
Nouanthavong S.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
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