สืบค้นงานวิจัย
Suppression of α-glucosidase gene by RNA interference can increase resistance of Culex quinquefasciatus to Bacillus sphaericus binary toxin
Woradulayapinij W. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Suppression of α-glucosidase gene by RNA interference can increase resistance of Culex quinquefasciatus to Bacillus sphaericus binary toxin
ผู้แต่ง / หัวหน้าโครงการ (EN): Woradulayapinij W.
บทคัดย่อ (EN): Bacillus sphaericus binary toxin displays a specific toxicity towards Culex spp. larvae. Resistance of Culex quinquefasciatus to B. sphaericus is associated with the reduction or absence of 66-kDa α-glucosidase, a binary toxin receptor, in larval midgut microvilli. In this study, long double-stranded RNA (dsRNA) targeting the α-glucosidase gene was used to suppress the expression of α-glucosidase in Cx. quinquefasciatus larvae and the consequences of the suppression was investigated. Three sets of dsRNAs, dsGRi1, dsGRi2, and dsGRi3, which are homologous to distinct regions of α-glucosidase gene, were delivered to mosquito larva by feeding with dsRNA–chitosan particles. Reverse transcriptase-polymerase chain reaction results demonstrated that the expression of α-glucosidase mRNA was reduced by 43% in larvae fed with dsGRi3–chitosan, compared to those fed with dsgfp–chitosan particles. Western blot analysis revealed a 7–17% reduction of α-glucosidase protein expression in larvae fed with dsGRi–chitosan. Mortality rates of larvae that received dsRNAs–chitosan particles were only 45–62% compared to those that received dsgfp–chitosan particles. These results indicate that α-glucosidase is essential for the toxicity of B. sphaericus binary toxin to Cx. quinquefasciatus. © 2015 Taylor & Francis.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-84944059456&doi=10.1080%2f09583157.2015.1066310&partnerID=40&md5=775094ce83cf059bc888e0262c4139f9
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Culex pipiens quinquefasciatus
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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การอ้างอิง

Woradulayapinij W.. "Suppression of α-glucosidase gene by RNA interference can increase resistance of Culex quinquefasciatus to Bacillus sphaericus binary toxin". ไม่ระบุหน่วยงาน. ม.ป.ป.

Woradulayapinij W.. "Suppression of α-glucosidase gene by RNA interference can increase resistance of Culex quinquefasciatus to Bacillus sphaericus binary toxin". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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Suppression of α-glucosidase gene by RNA interference can increase resistance of Culex quinquefasciatus to Bacillus sphaericus binary toxin
Woradulayapinij W.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
Lysinibacillus sphaericus binary toxin induces apoptosis in susceptible Culex quinquefasciatus larvae Development of larvicidal Bacillus thuringiensis var. israelensis by the Thai NIH and its comparison to Bacillus sphaericus and temephos in a selection experiment with the mosquito Culex quinquefascia Monitoring of resistance in laboratory susceptible strain and field population strains of Culex quinquefasciatus to Bacillus thuringiensis var. israelensis (Bti), Bacillus sphaericus (Bsph) and temeph Molecular analysis of Culex quinquefasciatus larvae responses to Lysinibacillus sphaericus bin toxin Interaction of Lysinibacillus sphaericus binary toxin with mosquito larval gut cells: Binding and internalization Evaluation of α-glucosidase inhibitory assay using different sub-classes of flavonoids Development of B-Soy starter for biolarvicide production for control of Culex quinquefasciatus mosquito larvae Blood-induced differential gene expression in Anopheles dirus evaluated using RNA sequencing Efficiency of Tinospora crispa against Culex quinquefasciatus larva Binary toxin subunits of lysinibacillus sphaericus are monomeric and form heterodimers after in vitro activation
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