สืบค้นงานวิจัย
A pathophysiological model of non-alcoholic fatty liver disease using precision-cut liver slices
Prins G.H. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): A pathophysiological model of non-alcoholic fatty liver disease using precision-cut liver slices
ผู้แต่ง / หัวหน้าโครงการ (EN): Prins G.H.
บทคัดย่อ (EN): Non-alcoholic fatty liver disease (NAFLD) is a common liver disorder closely related to metabolic syndrome. NAFLD can progress to an inflammatory state called non-alcoholic steatohepatitis (NASH), which may result in the development of fibrosis and hepatocellular carcinoma. To develop therapeutic strategies against NAFLD, a better understanding of the molecular mechanism is needed. Current in vitro NAFLD models fail to capture the essential interactions between liver cell types and often do not reflect the pathophysiological status of patients. To overcome limitations of commonly used in vitro and in vivo models, precision-cut liver slices (PCLSs) were used in this study. PCLSs, prepared from liver tissue obtained from male Wistar rats, were cultured in supraphysiological concentrations of glucose, fructose, insulin, and palmitic acid to mimic metabolic syndrome. Accumulation of lipid droplets was visible and measurable after 24 h in PCLSs incubated with glucose, fructose, and insulin, both in the presence and absence of palmitic acid. Upregulation of acetyl-CoA carboxylase 1 and 2, and of sterol responsive element binding protein 1c, suggests increased de novo lipogenesis in PCLSs cultured under these conditions. Additionally, carnitine palmitoyltransferase 1 expression was reduced, which indicates impaired fatty acid transport and disrupted mitochondrial β-oxidation. Thus, steatosis was successfully induced in PCLSs with modified culture medium. This novel ex vivo NAFLD model could be used to investigate the multicellular and molecular mechanisms that drive NAFLD development and progression, and to study potential anti-steatotic drugs. © 2019 by the authors. Licensee MDPI, Basel, Switzerland.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-85062398507&doi=10.3390%2fnu11030507&partnerID=40&md5=c06f95219848d1011957050c5971a35c
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): upregulation
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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Prins G.H.. "A pathophysiological model of non-alcoholic fatty liver disease using precision-cut liver slices". ไม่ระบุหน่วยงาน. ม.ป.ป.

Prins G.H.. "A pathophysiological model of non-alcoholic fatty liver disease using precision-cut liver slices". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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A pathophysiological model of non-alcoholic fatty liver disease using precision-cut liver slices
Prins G.H.
มหาวิทยาลัยมหิดล
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