สืบค้นงานวิจัย
Identification and Molecular Characterization of Multiple forms of â-1. 3-Glucanade Gene Promoter from Hevea brasiliensis through Inverse PCR
Supriya R. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Identification and Molecular Characterization of Multiple forms of â-1. 3-Glucanade Gene Promoter from Hevea brasiliensis through Inverse PCR
ผู้แต่ง / หัวหน้าโครงการ (EN): Supriya R.
บทคัดย่อ (EN): The first line of defense against fungal pathogens is performed by a group of pathogenesis-related proteins (PR), including â-1, 3-glucanased. Often these processes are controlled by transcriptional activation of the respective genes. In Hevea brasiliensis. â-1, 3-glucanase is playing a major role in combating abnormal leaf fall disease caused by Phytophthora spp. In the present study the inverse PCR technique, a method for the amplification of unknown flanking DNA sequences of a known genomic DNA sequence was optimixed and isolated the promoter sequences of â-1, 3-glucanase gene from H. brasiliensis. â-1, 3-glucanase gene specific forward and reverse inverse PCR primers were designed based on a previously reported â-1, 3-glucanaxe gene from a H. brasiliensis, clone RRII 105 (Thanseem et al. 2003 (NCBI#AY325498)). Under optimized conditions four bands of approzimately 1.2, 0.8, 0.76 and 0.4 kb were amplified. Nucleotide sequencing of the cloned bands revealed the presence of 1126, 793, 765 and 418 nucleotides. BLASTn analysis of these sequences showed>95% similarity with the reported H. brasiliensis â-1. 3-glucanase gene in the 5” UTR and the 5” coding region confirming that they are multiple forms of the â-1, 3-glucanase gene promoter. Excluding the known coding region, the fragments contain 913, 582, 553 and 198 nucleotides of the promoter region upstream to the translation initiation codon, ATG. Uut of these, the 198 bp promoter was exactly aligning with the reported promoter sequence by Thanseen et al. (2003) (AY325498). Although, the other three promoters showed similarity in the 5” UTR and immediate upstream region, they showed considerable variations in their distal regions. Analysis of the promoter sequences using ”PLACE” software detected important cis-elements that are usually present in biotic / abiotic stress related gene promoters. The presence of CpG islands and nucleosome forming potentials has also been identified in the isolated promoters. The properties of the isolated promoter regions of â-1, 3-glucanase gene on the basis of the data obtained is discussed. This is the first report on the existence of multiple forms of â-1, 3 glucanase gene promoters in the same clone of H. brasiliensis. After characterization of the pathogen inducible isoform, the same can be used for crop improvement through transgenic approaches as well as molecular breeding for disease tolerance.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เผยแพร่โดย (EN): การยางแห่งประเทศไทย
คำสำคัญ (EN): PCR
เจ้าของลิขสิทธิ์ (EN): การยางแห่งประเทศไทย
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การอ้างอิง

Supriya R.. "Identification and Molecular Characterization of Multiple forms of â-1. 3-Glucanade Gene Promoter from Hevea brasiliensis through Inverse PCR". ไม่ระบุหน่วยงาน. ม.ป.ป.

Supriya R.. "Identification and Molecular Characterization of Multiple forms of â-1. 3-Glucanade Gene Promoter from Hevea brasiliensis through Inverse PCR". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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Identification and Molecular Characterization of Multiple forms of â-1. 3-Glucanade Gene Promoter from Hevea brasiliensis through Inverse PCR
Supriya R.
การยางแห่งประเทศไทย
ไม่ระบุวันที่เผยแพร่
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