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A novel SNP (c.258+43C GT T) in LPL gene and association with milk production in buffaloes (Buffalo Bulletin,38(2))
Maryam Javed - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): A novel SNP (c.258+43C GT T) in LPL gene and association with milk production in buffaloes (Buffalo Bulletin,38(2))
ผู้แต่ง / หัวหน้าโครงการ (EN): Maryam Javed
หน่วยงานสังกัดผู้แต่ง (EN): Lahore (Pakistan). Institute of Biochemistry and Biotechnology
บทคัดย่อ (EN): Buffalo milk is the most preferred commodity in Pakistan due to high fat contents. Hydrolysis of circulating triglycerides and uptake of fatty acids in the mammary gland is regulated by lipoprotein lipase (LPL). This study is designed to identify single nucleotide polymorphisms (SNPs) in LPL gene among high and low milk producing buffalo breeds of Pakistan. We selected samples (n=50) of each Nili-Ravi a high milk producing and Azakheli a low milk producing buffalo breeds. Blood samples were collected for DNA extraction. LPL region of exon 2 region along with exon/intron boundaries were sequenced and data was analyzed for variation detection. Allele frequency was calculated using Hardy-Weinberg equation and in-silico analysis was performed for functional prediction and genetic diversity assessment. We found one single nucleotide polymorphism c.258+43C GT T in the intronic region of LPL gene. This polymorphism followed the Hardy-Weinberg equilibrium in both Nili-Ravi (P-value = 0.538) and Azakheli (P-value = 0.077). Association of T-allele analysis predicted it significantly higher (P-value = 0.009) in Nili-Ravi. We found one novel SNP (c.258+43C GT T) in the LPL gene in both high and low milk producing buffalo breeds of Pakistan but high in Nili-Ravi. Lower splice site effect suggests its less strength of association with milk producing trait.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://kukr.lib.ku.ac.th/db/kukr/search_detail/result/389074
คำสำคัญ (EN): Pakistan
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A novel SNP (c.258+43C GT T) in LPL gene and association with milk production in buffaloes (Buffalo Bulletin,38(2))
Maryam Javed
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DGAT1 gene polymorphisms and its association with milk production traits in Mehsana buffalo (Bubalus bubalis) (Buffalo Bulletin (Thailand),35(2)) Onion toxicosis in buffaloes (Buffalo Bulletin,38(2)) Production of buffalo milk (Bubalus bubalis) in Brazil (Buffalo Bulletin,39(3)) Milk lactoferrin concentrations in Anatolian buffaloes with and without subclinical mastitis (Buffalo Bulletin,38(2)) Sustainability of milk production in Surti buffalo on an organized farm (Buffalo Bulletin,40(1)) Histopathological study of endometritis in slaughtered buffaloes (Buffalo Bulletin,38(4)) Forecasting buffalo milk production in India: Time series approach (Buffalo Bulletin,40(2)) Comparative energetic and economic efficiency of indigenous and improved breed of buffalo and cattle for milk production in eastern gangetic plains (Buffalo Bulletin,38(3)) Water buffalo (Bubalus bubalis) susceptibility to bovine tuberculosis is influenced by g.4002c GT t polymorphism in interleukin-10 gene (Buffalo Bulletin,38(1)) Hydrallantois in buffaloes (Buffalo Bulletin,37(3))
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