สืบค้นงานวิจัย
Antibody-conjugated ferromagnetic nanoparticles with lateral flow test strip assay for rapid detection of Campylobacter jejuni in poultry samples
Poonlapdecha W. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Antibody-conjugated ferromagnetic nanoparticles with lateral flow test strip assay for rapid detection of Campylobacter jejuni in poultry samples
ผู้แต่ง / หัวหน้าโครงการ (EN): Poonlapdecha W.
บทคัดย่อ (EN): The aim of this study was to develop a nanoparticle-based cell capture system combined with a lateral flow test strip (LFT) assay for rapid detection of Campylobacter jejuni from poultry samples. The developed assay was bench-marked against the standard modified Charcoal Cefoperazone Deoxycholate Agar (mCCDA) method according to ISO16140:2003 procedures. The synthesized ferromagnetic nanoparticles (FMNs) were modified with glutaraldehyde, then functionalized with polyclonal antibodies for specific C. jejuni capture and concentration from poultry samples. After lysing captured cells, DNA from C. jejuni was amplified by PCR using the primers designed to target the hipO gene, and the PCR amplicons were detected with the lateral flow test strip assay. Following the ISO16140:2003 guidelines, the relative detection limit, and the inclusivity and exclusivity tests were determined. The results showed that the limit of detection (LOD) of the assay was 10 0 or 1 cfu/ml with C. jejuni in pure culture and 10 1 –10 2 cfu/ml with target cells spiked in poultry sample. In addition, the inclusivity and exclusivity tests were found to be 100%. Using field chicken samples (n = 60), the assay showed relative accuracy, relative specificity, and relative sensitivity of 96.67%, 100% and 93.33%, respectively. The positive predictive values (PPV) and negative predictive values (NPV), and the kappa index of concordance (k) were calculated as 100% and 93.75%, and 0.93, respectively. The developed assay required approximately 3 h to complete and gave results comparable to those analyzed by the standard culture method, which required 5–7 days. The assay is rapid, easy-to-use, and has potential to be directly applied to C. jejuni detection in various categories of poultry samples. © 2018
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-85049957179&doi=10.1016%2fj.ijfoodmicro.2018.07.009&partnerID=40&md5=e09183dcaaca2116fdee0a91e492bc3f
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Poultry
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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Poonlapdecha W.. "Antibody-conjugated ferromagnetic nanoparticles with lateral flow test strip assay for rapid detection of Campylobacter jejuni in poultry samples". ไม่ระบุหน่วยงาน. ม.ป.ป.

Poonlapdecha W.. "Antibody-conjugated ferromagnetic nanoparticles with lateral flow test strip assay for rapid detection of Campylobacter jejuni in poultry samples". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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Antibody-conjugated ferromagnetic nanoparticles with lateral flow test strip assay for rapid detection of Campylobacter jejuni in poultry samples
Poonlapdecha W.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
Rapid detection of Campylobacter jejuni, Campylobacter coli, and Campylobacter lari in fresh chicken meat and by-products in Bangkok, Thailand, using modified multiplex PCR Rapid molecular detection of multidrug- resistant tuberculosis by PCR-nucleic acid lateral flow immunoassay Development of botulinum toxin detection assay by antibody capture ELISA Development of the Rapid Test Kit for the Identification of Campylobacter spp. Based on Loop-mediated Isothermal Amplification (LAMP) in Combination with a Lateral Flow Dipstick (LFD) and Gold Nano-DN Sensitivity improvement of immunochromatographic strip test for infectious myonecrosis virus detection Detection of Campylobacter DNA using magnetic nanoparticles coupled with PCR and a colorimetric end-point system Development of competitive lateral flow immunoassay coupled with silver enhancement for simple and sensitive salivary cortisol detection Evaluation of HBsAg rapid test kits in Thailand Flow field-flow fractionation for particle size characterization of selenium nanoparticles incubated in gastrointestinal conditions การตรวจ XDR-TB ได้ผลเร็วด้วยวิธี Line Probe Assay Rapid XDR-TB detection using Line Probe Assay
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